2xcj

Crystal structure of P2 C, the immunity repressor of temperate E. coli phage P2

spinqualitylabelsall models 2xcj, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xcj is a 2 chain structure with sequence from Bpp2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FMT, GOL, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 A. This constitutes the first structure solved from the family of C proteins from P2-like bacteriophages. The structure reveals that the P2 C protein forms a symmetric dimer oriented to bind the major groove of two consecutive turns of the DNA. Surprisingly, P2 C has great similarities to binders of palindromic sequences. Nevertheless, the two identical DNA-binding helixes of the symmetric P2 C dimer have to bind different DNA sequences. Helix 3 is identified as the DNA-recognition motif in P2 C by alanine scanning and the importance for the individual residues in DNA recognition is defined. A truncation mutant shows that the disordered C-terminus is dispensable for repressor function. The short distance between the DNA-binding helices together with a possible interaction between two P2 C dimers are proposed to be responsible for extensive bending of the DNA. The structure provides insight into the mechanisms behind the mutants of P2 C causing dimer disruption, temperature sensitivity and insensitivity to the P4 antirepressor. Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.,Massad T, Skaar K, Nilsson H, Damberg P, Henriksson-Peltola P, Haggard-Ljungquist E, Hogbom M, Stenmark P Nucleic Acids Res. 2010 Jul 17. PMID:20639540 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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