User:Wally Novak/Sandbox Whitaker: Difference between revisions

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You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.
You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue.


== Function ==
==Function==
The U5 snRNA loop 1 interacts directly with the 5’ exon before the first step of splicing and with the 5’ and 3’ exons following the first step of splicing. These U5-exon interactions are essential for tethering and aligning the exons for ligation during the second step of splicing.
===General Overview===
The U5 snRNP consists of several proteins which are highly conserved from yeast to humans. These proteins consist of Prp8, Snu114, Brr2, Prp28, Snu40/52K, and Dib1 <ref>PMID:11720284</ref> (Stevens et al., 2001), however, only Prp8, Snu114, and Brr2 remain in complex with the U5 snRNA while the spliceosome is activated (Fabrizio et al., 2009). These proteins are essential for the remodeling of the spliceosome and may play a role in maintaining catalytic activity at the active center of the spliceosome. Assembly of the U5 snRNP is followed by the formation of the U4/U6.U5 tri-snRNP which is the last remaining complex necessary to form the complete spliceosome and is therefore vital for its function.  
 
== Structural Highlights ==
== Structural Highlights ==
By deletion analysis of the S. cerevisiae snRNA, we have demonstrated that the minimal U5 snRNA that can complement the lethal phenotype of a U5 gene disruption consists of (i) stem-loop 1, (ii) internal loop 1, (iii) a stem-closing internal loop 1, and (iv) the conserved Sm protein binding site
By deletion analysis of the S. cerevisiae snRNA, we have demonstrated that the minimal U5 snRNA that can complement the lethal phenotype of a U5 gene disruption consists of (i) stem-loop 1, (ii) internal loop 1, (iii) a stem-closing internal loop 1, and (iv) the conserved Sm protein binding site
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