6hsv: Difference between revisions

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'''Unreleased structure'''


The entry 6hsv is ON HOLD
==Engineered higher-order assembly of Cholera Toxin B subunits via the addition of C-terminal parallel coiled-coiled domains==
<StructureSection load='6hsv' size='340' side='right'caption='[[6hsv]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6hsv]] is a 20 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HSV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HSV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hsv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hsv OCA], [http://pdbe.org/6hsv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hsv RCSB], [http://www.ebi.ac.uk/pdbsum/6hsv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hsv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The self-assembly of proteins into higher order structures is ubiquitous in living systems. It is also an essential process for the bottom-up creation of novel molecular architectures and devices for synthetic biology. However, the complexity of protein-protein interaction surfaces makes it challenging to mimic natural assembly processes in artificial systems. Indeed, many successful computationally designed protein assemblies are pre-screened for 'designability', limiting the choice of components. Here, we report a simple and pragmatic strategy to assemble chosen multi-subunit proteins into more complex structures. A coiled-coil domain appended to one face of the pentameric cholera toxin B-subunit (CTB) enabled the ordered assembly of tubular supra-molecular complexes. X-ray crystallography and analysis of a tubular structure has revealed a hierarchical assembly process that displays features reminiscent of the polymorphic assembly of polyomavirus proteins. The approach provides a simple and straightforward method to direct the assembly of protein building blocks which present either termini on a single face of an oligomer. This scaffolding approach can be used to generate bespoke supramolecular assemblies of functional proteins. Additionally, structural resolution of the scaffolded assemblies highlight 'native-state' forced protein-protein interfaces, which may prove useful as starting conformations for future computational design.


Authors: Pearson, A.R., Turnbull, W.B., Ross, J.F., Trinh, C.H., Webb, M.E.
Directed assembly of homo-pentameric cholera toxin B-subunit proteins into higher-order structures using coiled-coil appendages.,Ross JF, Wildsmith GC, Johnson M, Hurdiss D, Hollingsworth K, Thompson RF, Mosayebi M, Trinh CH, Paci E, Pearson AR, Webb ME, Turnbull WB J Am Chem Soc. 2019 Mar 11. doi: 10.1021/jacs.8b11480. PMID:30856321<ref>PMID:30856321</ref>


Description: Engineered higher-order assembly of Cholera Toxin B subunits via the addition of C-terminal parallel coiled-coiled domains
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Pearson, A.R]]
<div class="pdbe-citations 6hsv" style="background-color:#fffaf0;"></div>
[[Category: Turnbull, W.B]]
== References ==
[[Category: Webb, M.E]]
<references/>
[[Category: Ross, J.F]]
__TOC__
[[Category: Trinh, C.H]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Pearson, A R]]
[[Category: Ross, J F]]
[[Category: Trinh, C H]]
[[Category: Turnbull, W B]]
[[Category: Webb, M E]]
[[Category: Cholera toxin b]]
[[Category: Ctb]]
[[Category: Designed protein]]
[[Category: Higher order assembly]]
[[Category: Nanotube]]
[[Category: Parallel coiled coil]]
[[Category: Self assembly]]
[[Category: Toxin]]
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