2qvw: Difference between revisions
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'''Structure of Giardia Dicer refined against twinned data''' | '''Structure of Giardia Dicer refined against twinned data''' | ||
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[[Category: Doudna, J A.]] | [[Category: Doudna, J A.]] | ||
[[Category: MacRae, I J]] | [[Category: MacRae, I J]] | ||
[[Category: | [[Category: Dicer]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Rnai,pseudo-merohedral twin]] | ||
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Revision as of 12:47, 4 May 2008
Structure of Giardia Dicer refined against twinned data
Overview
The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.
About this Structure
2QVW is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.
Reference
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer., MacRae IJ, Doudna JA, Acta Crystallogr D Biol Crystallogr. 2007 Sep;63(Pt 9):993-9. Epub 2007, Aug 17. PMID:17704568 Page seeded by OCA on Sun May 4 15:47:02 2008
