5l93

An atomic model of HIV-1 CA-SP1 reveals structures regulating assembly and maturation

spinqualitylabelsall models 5l93, resolution 3.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5l93 is a 3 chain structure with sequence from Hiv-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: gag (HIV-1) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [GAG_HV1N5] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity). Publication Abstract from PubMed Immature HIV-1 assembles at, and buds from, the plasma membrane before proteolytic cleavage of the viral Gag polyprotein induces structural maturation. Maturation is blocked by protease and maturation inhibitors (MIs), abolishing infectivity. The CA (capsid) and SP1 (spacer peptide 1) region of Gag is the key regulator of assembly and maturation, and the target of MIs. Here we applied optimized cryo-electron tomography and subtomogram averaging to resolve this region within assembled immature HIV-1 particles at 3.9 A resolution and built an atomic model. The structure reveals a network of intra- and inter-molecular interactions mediating immature HIV-1 assembly. The proteolytic cleavage site between CA and SP1 is inaccessible to protease. We suggest that MIs prevent CA-SP1 cleavage by stabilizing the structure, and MI resistance develops by destabilizing CA-SP1. An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation.,Schur FK, Obr M, Hagen WJ, Wan W, Jakobi AJ, Kirkpatrick JM, Sachse C, Krausslich HG, Briggs JA Science. 2016 Jul 14. pii: aaf9620. PMID:27417497 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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