5zza

OdinProfilin/Rabbit Actin Complex

spinqualitylabelsall models 5zza, resolution 1.53Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5zza is a 2 chain structure with sequence from Candidatus Odinarchaeota archaeon LCB_4 and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.53Å Ligands: ATP, CA, HIC, LAB Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PROF_ODILC Binds to actin and affects the structure of the cytoskeleton. At high concentrations inhibits spontaneous rabbit actin nucleation. This strongly suggests this archaea has a profilin-regulated actin system, and actin-type genes can be identified in this organism. Publication Abstract from PubMed The origin of the eukaryotic cell is unresolved(1,2). Metagenomics sequencing has recently identified several potential eukaryotic gene homologues in Asgard archaea(3,4), consistent with the hypothesis that the eukaryotic cell evolved from within the Archaea domain. However, many of these eukaryotic-like sequences are highly divergent and the organisms have yet to be imaged or cultivated, which brings into question the extent to which these archaeal proteins represent functional equivalents of their eukaryotic counterparts. Here we show that Asgard archaea encode functional profilins and thereby establish that this archaeal superphylum has a regulated actin cytoskeleton, one of the hallmarks of the eukaryotic cell(5). Loki profilin-1, Loki profilin-2 and Odin profilin adopt the typical profilin fold and are able to interact with rabbit actin-an interaction that involves proteins from species that diverged more than 1.2 billion years ago(6). Biochemical experiments reveal that mammalian actin polymerizes in the presence of Asgard profilins; however, Loki, Odin and Heimdall profilins impede pointed-end elongation. These archaeal profilins also retard the spontaneous nucleation of actin filaments, an effect that is reduced in the presence of phospholipids. Asgard profilins do not interact with polyproline motifs and the profilin-polyproline interaction therefore probably evolved later in the Eukarya lineage. These results suggest that Asgard archaea possess a primordial, polar, profilin-regulated actin system, which may be localized to membranes owing to the sensitivity of Asgard profilins to phospholipids. Because Asgard archaea are also predicted to encode potential eukaryotic-like genes involved in membrane-trafficking and endocytosis(3,4), imaging is now necessary to elucidate whether these organisms are capable of generating eukaryotic-like membrane dynamics that are regulated by actin, such as are observed in eukaryotic cell movement, podosomes and endocytosis. Genomes of Asgard archaea encode profilins that regulate actin.,Akil C, Robinson RC Nature. 2018 Oct 3. pii: 10.1038/s41586-018-0548-6. doi:, 10.1038/s41586-018-0548-6. PMID:30283132 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Actin 3D structures

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