DNA Origami Assembly for the Tar Chemoreceptor: Difference between revisions
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==Attachment to DNA== | ==Attachment to DNA== | ||
The protein receptor dimer is attached to the DNA tetrahedron using NTA-functionalized DNA. This means that the DNA has an NTA, or nitrilotriaceticacid, is able to coordinate with nickel ions, shown in green, which is also able to coordinate with histidines. The Tar chemoreceptor has six histidines added to the N-terminus of the protein ''in vitro'', which should be able to coordinate with the nickel ion as well, creating a coordination complex. | The protein receptor dimer is attached to the DNA tetrahedron using NTA-functionalized DNA. This means that the DNA has an NTA, or nitrilotriaceticacid, is able to coordinate with nickel ions, shown in green, which is also able to coordinate with histidines. The Tar chemoreceptor has six histidines added to the N-terminus of the protein ''in vitro'', which should be able to coordinate with the nickel ion as well, creating a coordination complex. | ||
<scene name='80/800127/Zoomed_in_connection/1'>DNA-protein linkage</scene> | |||
== Structural highlights == | == Structural highlights == | ||
