6i27: Difference between revisions
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The | ==Rea1 AAA2L-H2alpha deletion mutant in AMPPNP State== | ||
<StructureSection load='6i27' size='340' side='right' caption='[[6i27]], [[Resolution|resolution]] 7.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6i27]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I27 FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6hyd|6hyd]], [[6hyp|6hyp]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i27 OCA], [http://pdbe.org/6i27 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i27 RCSB], [http://www.ebi.ac.uk/pdbsum/6i27 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i27 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The biogenesis of 60S ribosomal subunits is initiated in the nucleus where rRNAs and proteins form pre-60S particles. These pre-60S particles mature by transiently interacting with various assembly factors. The ~5000 amino-acid AAA+ ATPase Rea1 (or Midasin) generates force to mechanically remove assembly factors from pre-60S particles, which promotes their export to the cytosol. Here we present three Rea1 cryoEM structures. We visualise the Rea1 engine, a hexameric ring of AAA+ domains, and identify an alpha-helical bundle of AAA2 as a major ATPase activity regulator. The alpha-helical bundle interferes with nucleotide-induced conformational changes that create a docking site for the substrate binding MIDAS domain on the AAA +ring. Furthermore, we reveal the architecture of the Rea1 linker, which is involved in force generation and extends from the AAA+ ring. The data presented here provide insights into the mechanism of one of the most complex ribosome maturation factors. | |||
The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.,Sosnowski P, Urnavicius L, Boland A, Fagiewicz R, Busselez J, Papai G, Schmidt H Elife. 2018 Nov 26;7. pii: 39163. doi: 10.7554/eLife.39163. PMID:30460895<ref>PMID:30460895</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6i27" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Boland, A]] | [[Category: Boland, A]] | ||
[[Category: Busselez, J]] | |||
[[Category: Fagiewicz, R]] | [[Category: Fagiewicz, R]] | ||
[[Category: | [[Category: Papai, G]] | ||
[[Category: Schmidt, H]] | [[Category: Schmidt, H]] | ||
[[Category: Sosnowski, P]] | [[Category: Sosnowski, P]] | ||
[[Category: Urnavicius, L]] | |||
[[Category: Aaa+ protein]] | |||
[[Category: Mdn1]] | |||
[[Category: Midasin]] | |||
[[Category: Molecular machine]] | |||
[[Category: Motor protein]] | |||
[[Category: Rea1]] | |||
[[Category: Ribosome maturation]] | |||
Revision as of 06:52, 12 December 2018
Rea1 AAA2L-H2alpha deletion mutant in AMPPNP State
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