6fsx: Difference between revisions

Latest revision as of 12:30, 9 May 2024

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

Structural highlights

6fsx is a 2 chain structure with sequence from Rhodopseudomonas palustris CGA009. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DEHA_RHOPA Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.^[1] ^[2]

Publication Abstract from PubMed

We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.,Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chan PW, Yakunin AF, Edwards EA, Pai EF. Mapping the Reaction Coordinates of Enzymatic Defluorination. J Am Chem Soc. 2011 Apr 21. PMID:21510690 doi:10.1021/ja200277d
↑ Chan PW, Yakunin AF, Edwards EA, Pai EF. Mapping the Reaction Coordinates of Enzymatic Defluorination. J Am Chem Soc. 2011 Apr 21. PMID:21510690 doi:10.1021/ja200277d
↑ Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD. The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366 doi:https://dx.doi.org/10.1038/s41592-018-0180-2

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Chan PW, Yakunin AF, Edwards EA, Pai EF. Mapping the Reaction Coordinates of Enzymatic Defluorination. J Am Chem Soc. 2011 Apr 21. PMID:21510690 doi:10.1021/ja200277d

[2] Chan PW, Yakunin AF, Edwards EA, Pai EF. Mapping the Reaction Coordinates of Enzymatic Defluorination. J Am Chem Soc. 2011 Apr 21. PMID:21510690 doi:10.1021/ja200277d

[3] Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD. The hit-and-return system enables efficient time-resolved serial synchrotron crystallography. Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366 doi:https://dx.doi.org/10.1038/s41592-018-0180-2

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==The hit-and-return system enables efficient time-resolved serial synchrotron crystallography==
-<StructureSection load='6fsx' size='340' side='right' caption='[[6fsx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
+<StructureSection load='6fsx' size='340' side='right'caption='[[6fsx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6fsx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopa Rhopa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FSX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FSX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6fsx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris_CGA009 Rhodopseudomonas palustris CGA009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FSX FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5o2h|5o2h]], [[5o2i|5o2i]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPA1163 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=258594 RHOPA])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fsx OCA], [https://pdbe.org/6fsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fsx RCSB], [https://www.ebi.ac.uk/pdbsum/6fsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fsx ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Haloacetate_dehalogenase Haloacetate dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.3 3.8.1.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fsx OCA], [http://pdbe.org/6fsx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fsx RCSB], [http://www.ebi.ac.uk/pdbsum/6fsx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fsx ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DEHA_RHOPA DEHA_RHOPA]] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.<ref>PMID:21510690</ref> <ref>PMID:21510690</ref>
+[https://www.uniprot.org/uniprot/DEHA_RHOPA DEHA_RHOPA] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate.<ref>PMID:21510690</ref> <ref>PMID:21510690</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 18: @@
 </div>
 <div class="pdbe-citations 6fsx" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Haloacetate dehalogenase]]
+[[Category: Large Structures]]
-[[Category: Rhopa]]
+[[Category: Rhodopseudomonas palustris CGA009]]
-[[Category: Diederich, F]]
+[[Category: De Gasparo R]]
-[[Category: Gasparo, R De]]
+[[Category: Diederich F]]
-[[Category: Jha, A]]
+[[Category: Jha A]]
-[[Category: Mehrabi, P]]
+[[Category: Mehrabi P]]
-[[Category: Miller, D]]
+[[Category: Miller D]]
-[[Category: Mueller-werkmeiser, H]]
+[[Category: Mueller-werkmeiser H]]
-[[Category: Pai, E]]
+[[Category: Pai E]]
-[[Category: Persch, E]]
+[[Category: Persch E]]
-[[Category: Schulz, E C]]
+[[Category: Schulz EC]]
-[[Category: Stuart, W]]
+[[Category: Stuart W]]
-[[Category: Tellkamp, F]]
+[[Category: Tellkamp F]]
-[[Category: Halogenase]]
-[[Category: Hydrolase]]

6fsx: Difference between revisions

Latest revision as of 12:30, 9 May 2024

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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