5vl9

Crystal structure of EilR in complex with eilO DNA element

spinqualitylabelsall models 5vl9, resolution 2.16Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5vl9 is a 8 chain structure with sequence from "enterobacter_lignolyticus"_deangelis_et_al._2011 "enterobacter lignolyticus" deangelis et al. 2011. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HEZ NonStd Res: MSE Related: 5vlm, 5vlg Gene: Entcl_2353 ("Enterobacter lignolyticus" Deangelis et al. 2011) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability are desirable. Current systems are often incompatible with large-scale fermentations due to high inducer costs and strict media requirements. Here, we describe the bottom-up engineering of 'Jungle Express', an expression system that enables efficient gene regulation in diverse proteobacteria. This system is guided by EilR, a multidrug-binding repressor with high affinity to its optimized operator and cationic dyes that act as powerful inducers at negligible costs. In E. coli, the engineered promoters exhibit minimal basal transcription and are inducible over four orders of magnitude by 1 microM crystal violet, reaching expression levels exceeding those of the strongest current bacterial systems. Further, we provide molecular insights into specific interactions of EilR with its operator and with two inducers. The versatility of Jungle Express opens the way for tightly controlled and efficient gene expression that is not restricted to host organism, substrate, or scale. Jungle Express is a versatile repressor system for tight transcriptional control.,Ruegg TL, Pereira JH, Chen JC, DeGiovanni A, Novichkov P, Mutalik VK, Tomaleri GP, Singer SW, Hillson NJ, Simmons BA, Adams PD, Thelen MP Nat Commun. 2018 Sep 6;9(1):3617. doi: 10.1038/s41467-018-05857-3. PMID:30190458 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA