Sandbox Reserved 1477: Difference between revisions
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[[Image:Position of FDA of GOX from PA.png]] | [[Image:Position of FDA of GOX from PA.png]] | ||
Fig. 2 Relative position of two FAD molecules of the penicillium amagasakiense (PDB code: 1gpe) with transparent surface, which will hopefully give a rough image about how the two chains of the enzyme are arranged. Since the 1cf3 file (GOX from aspergillus niger) only have one chain given, the 1gpe (GOX from penicillium amagasakiense) was used instead. | Fig. 2 Relative position of two FAD molecules of the penicillium amagasakiense (PDB code: 1gpe) with transparent surface, which will hopefully give a rough image about how the two chains of the enzyme are arranged. Since the 1cf3 file (GOX from aspergillus niger) only have one chain given, the 1gpe (GOX from penicillium amagasakiense) was used instead. | ||
12 hydrogen bonds formed with the glucose (9 with residues, 2 with water molecules and 1 with the cofactor) as well as the hydrophobic effect of Phe-418 and Trp-430 stabilize the active site of the GOX <ref>PMID:10749686</ref>. As mentioned, the critical hydrogen bonds are the three hydrogen bonds formed between Arg-516 and 3-OH of the glucose based on the discussion of the function part. | |||
[[Media:Movie compressed compressed.mp4]] | [[Media:Movie compressed compressed.mp4]] | ||
