5z7g: Difference between revisions

Revision as of 21:15, 28 October 2020

Crystal structure of TAX1BP1 SKICH region in complex with NAP1

Structural highlights

5z7g is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	GOL
Related:	5z7a
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TAXB1_HUMAN] Inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degraded by caspase-3-like family proteins upon TNF-induced apoptosis. May also play a role in the pro-inflammatory cytokine IL-1 signaling cascade.^[1] ^[2] [AZI2_HUMAN] Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.^[3] ^[4] ^[5]

Publication Abstract from PubMed

NDP52 and TAX1BP1, two SKIP carboxyl homology (SKICH) domain-containing autophagy receptors, play crucial roles in selective autophagy. The autophagic functions of NDP52 and TAX1BP1 are regulated by TANK-binding kinase 1 (TBK1), which may associate with them through the adaptor NAP1. However, the molecular mechanism governing the interactions of NAP1 with NDP52 and TAX1BP1, as well as the effects induced by TBK1-mediated phosphorylation of NDP52 and TAX1BP1, remains elusive. Here, we report the atomic structures of the SKICH regions of NDP52 and TAX1BP1 in complex with NAP1, which not only uncover the mechanistic bases underpinning the specific interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1 but also reveal the binding mode of a SKICH domain. Moreover, we uncovered that the SKICH domains of NDP52 and TAX1BP1 share a general binding mode to interact with NAP1. Finally, we also evaluated the currently known TBK1-mediated phosphorylation sites in the SKICH domains of NDP52 and TAX1BP1 on the basis of their interactions with NAP1. In all, our findings provide mechanistic insights into the interactions of NAP1 with NDP52 and TAX1BP1, and are valuable for further understanding the functions of these proteins in selective autophagy.

Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1.,Fu T, Liu J, Wang Y, Xie X, Hu S, Pan L Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11651-E11660. doi:, 10.1073/pnas.1811421115. Epub 2018 Nov 20. PMID:30459273^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ De Valck D, Jin DY, Heyninck K, Van de Craen M, Contreras R, Fiers W, Jeang KT, Beyaert R. The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases. Oncogene. 1999 Jul 22;18(29):4182-90. PMID:10435631 doi:https://dx.doi.org/10.1038/sj.onc.1202787
↑ Ling L, Goeddel DV. T6BP, a TRAF6-interacting protein involved in IL-1 signaling. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9567-72. PMID:10920205 doi:https://dx.doi.org/10.1073/pnas.170279097
↑ Fujita F, Taniguchi Y, Kato T, Narita Y, Furuya A, Ogawa T, Sakurai H, Joh T, Itoh M, Delhase M, Karin M, Nakanishi M. Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling. Mol Cell Biol. 2003 Nov;23(21):7780-93. PMID:14560022
↑ Sasai M, Oshiumi H, Matsumoto M, Inoue N, Fujita F, Nakanishi M, Seya T. Cutting Edge: NF-kappaB-activating kinase-associated protein 1 participates in TLR3/Toll-IL-1 homology domain-containing adapter molecule-1-mediated IFN regulatory factor 3 activation. J Immunol. 2005 Jan 1;174(1):27-30. PMID:15611223
↑ Goncalves A, Burckstummer T, Dixit E, Scheicher R, Gorna MW, Karayel E, Sugar C, Stukalov A, Berg T, Kralovics R, Planyavsky M, Bennett KL, Colinge J, Superti-Furga G. Functional dissection of the TBK1 molecular network. PLoS One. 2011;6(9):e23971. doi: 10.1371/journal.pone.0023971. Epub 2011 Sep 8. PMID:21931631 doi:https://dx.doi.org/10.1371/journal.pone.0023971
↑ Fu T, Liu J, Wang Y, Xie X, Hu S, Pan L. Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11651-E11660. doi:, 10.1073/pnas.1811421115. Epub 2018 Nov 20. PMID:30459273 doi:https://dx.doi.org/10.1073/pnas.1811421115

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OCA

[1] De Valck D, Jin DY, Heyninck K, Van de Craen M, Contreras R, Fiers W, Jeang KT, Beyaert R. The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases. Oncogene. 1999 Jul 22;18(29):4182-90. PMID:10435631 doi:https://dx.doi.org/10.1038/sj.onc.1202787

[2] Ling L, Goeddel DV. T6BP, a TRAF6-interacting protein involved in IL-1 signaling. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9567-72. PMID:10920205 doi:https://dx.doi.org/10.1073/pnas.170279097

[3] Fujita F, Taniguchi Y, Kato T, Narita Y, Furuya A, Ogawa T, Sakurai H, Joh T, Itoh M, Delhase M, Karin M, Nakanishi M. Identification of NAP1, a regulatory subunit of IkappaB kinase-related kinases that potentiates NF-kappaB signaling. Mol Cell Biol. 2003 Nov;23(21):7780-93. PMID:14560022

[4] Sasai M, Oshiumi H, Matsumoto M, Inoue N, Fujita F, Nakanishi M, Seya T. Cutting Edge: NF-kappaB-activating kinase-associated protein 1 participates in TLR3/Toll-IL-1 homology domain-containing adapter molecule-1-mediated IFN regulatory factor 3 activation. J Immunol. 2005 Jan 1;174(1):27-30. PMID:15611223

[5] Goncalves A, Burckstummer T, Dixit E, Scheicher R, Gorna MW, Karayel E, Sugar C, Stukalov A, Berg T, Kralovics R, Planyavsky M, Bennett KL, Colinge J, Superti-Furga G. Functional dissection of the TBK1 molecular network. PLoS One. 2011;6(9):e23971. doi: 10.1371/journal.pone.0023971. Epub 2011 Sep 8. PMID:21931631 doi:https://dx.doi.org/10.1371/journal.pone.0023971

[6] Fu T, Liu J, Wang Y, Xie X, Hu S, Pan L. Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11651-E11660. doi:, 10.1073/pnas.1811421115. Epub 2018 Nov 20. PMID:30459273 doi:https://dx.doi.org/10.1073/pnas.1811421115

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@@ Line 1: / Line 1: @@
 ==Crystal structure of TAX1BP1 SKICH region in complex with NAP1==
-<StructureSection load='5z7g' size='340' side='right' caption='[[5z7g]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='5z7g' size='340' side='right'caption='[[5z7g]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z7g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z7G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z7g]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z7G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z7G FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5z7a|5z7a]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z7g OCA], [http://pdbe.org/5z7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z7g RCSB], [http://www.ebi.ac.uk/pdbsum/5z7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z7g ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z7g OCA], [http://pdbe.org/5z7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z7g RCSB], [http://www.ebi.ac.uk/pdbsum/5z7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z7g ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Fu, T]]
 [[Category: Hu, S C]]

5z7g: Difference between revisions

Revision as of 21:15, 28 October 2020

Crystal structure of TAX1BP1 SKICH region in complex with NAP1

Structural highlights

Function

Publication Abstract from PubMed

References

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