3ygs: Difference between revisions
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'''APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9''' | '''APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9''' | ||
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[[Category: Srinivasula, S.]] | [[Category: Srinivasula, S.]] | ||
[[Category: Wu, G.]] | [[Category: Wu, G.]] | ||
[[Category: | [[Category: Apoptosis]] | ||
[[Category: | [[Category: Caspase activation]] | ||
[[Category: | [[Category: Caspase recruitment]] | ||
[[Category: | [[Category: Recognition complex]] | ||
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Revision as of 19:15, 4 May 2008
APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9
Overview
Caspase-9-mediated apoptosis (programmed cell death) plays a central role in the development and homeostasis of all multicellular organisms. Mature caspase-9 is derived from its procaspase precursor as a result of recruitment by the activating factor Apaf-1. The crystal structures of the caspase-recruitment domain of Apaf-1 by itself and in complex with the prodomain of procaspase-9 have been determined at 1.6 and 2.5 A resolution, respectively. These structures and other evidence reveal that each molecule of Apaf-1 interacts with a molecule of procaspase-9 through two highly charged and complementary surfaces formed by non-conserved residues; these surfaces determine recognition specificity through networks of intermolecular hydrogen bonds and van der Waals interactions. Mutation of the important interface residues in procaspase-9 or Apaf-1 prevents or reduces activation of procaspase-9 in a cell-free system. Wild-type, but not mutant, prodomains of caspase-9 completely inhibit catalytic processing of procaspase-9. Furthermore, analysis of homologues from Caenorhabditis elegans indicates that recruitment of CED-3 by CED-4 is probably mediated by the same set of conserved structural motifs, with a corresponding change in the specificity-determining residues.
About this Structure
3YGS is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1., Qin H, Srinivasula SM, Wu G, Fernandes-Alnemri T, Alnemri ES, Shi Y, Nature. 1999 Jun 10;399(6736):549-57. PMID:10376594 Page seeded by OCA on Sun May 4 22:15:41 2008
