6qcc: Difference between revisions

<table><tr><td colspan='2'>[[6qcc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Broad_bean_stain_virus Broad bean stain virus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5npx 5npx]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QCC FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qcc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qcc OCA], [http://pdbe.org/6qcc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qcc RCSB], [http://www.ebi.ac.uk/pdbsum/6qcc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qcc ProSAT]</span></td></tr>

The Broad bean stain virus (BBSV) is a member of the genus Comovirus infecting Fabaceae. The virus is transmitted through seed and by plant weevils causing severe and widespread disease worldwide. BBSV has a bipartite, positive-sense, single-stranded RNA genome encapsidated in icosahedral particles. We present here the cryo-electron microscopy reconstruction of the BBSV and an atomic model of the capsid proteins refined at 3.22A resolution. We identified residues involved in RNA/capsid interactions revealing a unique RNA genome organization. Inspection of the small coat protein C-terminal domain highlights a maturation cleavage between Leu567 and Leu568 and interactions of the C-terminal stretch with neighbouring small coat proteins within the capsid pentameric turrets. These interactions previously proposed to play a key role in the assembly of the Cowpea mosaic virus suggest a common capsid assembly mechanism throughout all comovirus species.

The cryo-electron microscopy structure of Broad Bean Stain Virus suggests a common capsid assembly mechanism among comoviruses.,Lecorre F, Lai-Kee-Him J, Blanc S, Zeddam JL, Trapani S, Bron P Virology. 2019 Apr;530:75-84. doi: 10.1016/j.virol.2019.02.009. Epub 2019 Feb 13. PMID:30782565<ref>PMID:30782565</ref>

[[Category: Broad bean stain virus]]

[[Category: Large Structures]]