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==Crystal structure of StSPL in complex with phosphoethanolamine==
==Crystal structure of StSPL in complex with phosphoethanolamine==
<StructureSection load='3mau' size='340' side='right' caption='[[3mau]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
<StructureSection load='3mau' size='340' side='right'caption='[[3mau]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3mau]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_24528 Dsm 24528]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MAU FirstGlance]. <br>
<table><tr><td colspan='2'>[[3mau]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Symbiobacterium_thermophilum Symbiobacterium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MAU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3mad|3mad]], [[3maf|3maf]], [[3mc6|3mc6]], [[3mbb|3mbb]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EXT:{5-HYDROXY-6-METHYL-4-[(E)-{[2-(PHOSPHONOOXY)ETHYL]IMINO}METHYL]PYRIDIN-3-YL}METHYL+DIHYDROGEN+PHOSPHATE'>EXT</scene>, <scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">STH1274 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2734 DSM 24528])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mau OCA], [https://pdbe.org/3mau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mau RCSB], [https://www.ebi.ac.uk/pdbsum/3mau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mau ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sphinganine-1-phosphate_aldolase Sphinganine-1-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.27 4.1.2.27] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mau OCA], [http://pdbe.org/3mau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3mau RCSB], [http://www.ebi.ac.uk/pdbsum/3mau PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3mau ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q67PY4_SYMTH Q67PY4_SYMTH]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mau ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mau ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sphingosine-1-phosphate lyase (SPL), a key enzyme of sphingolipid metabolism, catalyzes the irreversible degradation of sphingoid base phosphates. Its main substrate sphingosine-1-phosphate (S1P) acts both extracellularly, by binding G protein-coupled receptors of the lysophospholipid receptor family, and inside the cell, as a second messenger. There, S1P takes part in regulating various cellular processes and its levels are tightly regulated. SPL is a pivotal enzyme regulating S1P intracellular concentrations and a promising drug target for the design of immunosuppressants. We structurally and functionally characterized yeast SPL (Dpl1p) and its first prokaryotic homolog, from Symbiobacterium thermophilum. The Dpl1p structure served as a basis for a very reliable model of Homo sapiens SPL. The above results, together with in vitro and in vivo studies of SPL mutants, reveal which residues are involved in activity and substrate binding and pave the way to studies aimed at controlling the activity of this pivotal enzyme.
Structure and function of sphingosine-1-phosphate lyase, a key enzyme of sphingolipid metabolism.,Bourquin F, Riezman H, Capitani G, Grutter MG Structure. 2010 Aug 11;18(8):1054-65. PMID:20696404<ref>PMID:20696404</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3mau" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Dsm 24528]]
[[Category: Large Structures]]
[[Category: Sphinganine-1-phosphate aldolase]]
[[Category: Symbiobacterium thermophilum]]
[[Category: Bourquin, F]]
[[Category: Bourquin F]]
[[Category: Capitani, G]]
[[Category: Capitani G]]
[[Category: Grutter, M G]]
[[Category: Grutter MG]]
[[Category: Carboxy-lyase activity]]
[[Category: Lyase]]
[[Category: Pyridoxyl phosphate]]

Revision as of 10:24, 21 February 2024

Crystal structure of StSPL in complex with phosphoethanolamine

3mau, resolution 2.90Å

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