Sandbox Reserved 1501: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Clara Nowak (talk | contribs) No edit summary |
Clara Nowak (talk | contribs) No edit summary |
||
| Line 10: | Line 10: | ||
Flavocytochrome b(2) (Arg289Lys mutant) from ''Saccharomyces cerevisiae'' is an oxidoreductase that couples dehydrogenation of L-lactate to cytochrome c reduction by electron transfer. It is one step of the bacterial lactate metabolic pathway. | Flavocytochrome b(2) (Arg289Lys mutant) from ''Saccharomyces cerevisiae'' is an oxidoreductase that couples dehydrogenation of L-lactate to cytochrome c reduction by electron transfer. It is one step of the bacterial lactate metabolic pathway. | ||
== Reaction == | |||
[[Image:Reaction1.JPG]] | |||
Similar oxidants that can be used to perform the reaction in vitro are ferricyanide, phenozine methosulfate and quinone [experiment first performed 1963 by Nygaard, later in 1966 described by Symons and Burgoyne]. | Similar oxidants that can be used to perform the reaction in vitro are ferricyanide, phenozine methosulfate and quinone [experiment first performed 1963 by Nygaard, later in 1966 described by Symons and Burgoyne]. | ||
The Yeasts L-Lactate Dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids [Nygaard 1963]. | The Yeasts L-Lactate Dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids [Nygaard 1963]. | ||
