Sandbox Reserved 1508: Difference between revisions

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The cysteine synthase (CysM) of Mycobacterium tuberculosis is an ''O''-Phosphoserine Sulfhydrylase (Ägren et al, 2008). The metabolic pathways of biosynthesis of cysteine are important for the synthesis of de novo proteins and for reduced thiol as a component of the oxidative defense mechanisms. That occurs in the dormant state of ''Mycobacterium tuberculosis''.

The cysteine synthase CysM is a O-phosphoserine-specific cysteine synthase which belongs to the fold type II pyridoxal 5’-phosphate-dependant enzymes (Ägren et al, 2008). The side chain of Arg220 interacts with the phosphate group by an hydrogen bound allowing the O-phosphoserine bound to the enzyme.

The cysteine synthase CysM is a ''O''-phosphoserine-specific cysteine synthase which belongs to the fold type II pyridoxal 5’-phosphate-dependant enzymes (Ägren et al, 2008). The side chain of Arg220 interacts with the phosphate group by an hydrogen bound allowing the ''O''-phosphoserine bound to the enzyme.

Thus, the signaling pathway of the cysteine synthase (CysM) of Mycobacterium tuberculosis leads an O-Phosphoserine binding with the enzyme O-Phosphoserine Sulfhydrylase. This mechanism is independent from the O-acetylserine and sulfate reduction pathway.

Thus, the signaling pathway of the cysteine synthase (CysM) of ''Mycobacterium tuberculosis'' leads an ''O''-Phosphoserine binding with the enzyme ''O''-Phosphoserine Sulfhydrylase. This mechanism is independent from the ''O''-acetylserine and sulfate reduction pathway.

According to researches on biosynthetic pathway in Mycobacterium tuberculosis (Burns et al, 2008), there are three pathways implicated cysteine in this disease: the sulfide dependent pathway, the cystathionine pathway and the CysO-thiocarboxylate pathway. For the CysO depending pathway, transcriptional profile analysis shown that cysM and cysO are upregulated under oxidative stress conditions. Moreover, the thiocarboxylate are much more resistant to oxidation than thiols. Thus, when the disease occurs, the environment becomes highly oxidizing due to the macrophages, leads to the cysteine biosynthesis. The CysO-thiocarboxylate evolves as an oxidation resistant form of sulfide, thiol is favored for the cysteine biosynthesis.

According to researches on biosynthetic pathway in ''Mycobacterium tuberculosis'' (Burns et al, 2008), there are three pathways implicated cysteine in this disease: the sulfide dependent pathway, the cystathionine pathway and the CysO-thiocarboxylate pathway. For the CysO depending pathway, transcriptional profile analysis shown that cysM and cysO are upregulated under oxidative stress conditions. Moreover, the thiocarboxylate are much more resistant to oxidation than thiols. Thus, when the disease occurs, the environment becomes highly oxidizing due to the macrophages, leads to the cysteine biosynthesis. The CysO-thiocarboxylate evolves as an oxidation resistant form of sulfide, thiol is favored for the cysteine biosynthesis.

Sandbox Reserved 1508: Difference between revisions

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 The cysteine synthase (CysM) of Mycobacterium tuberculosis is an ''O''-Phosphoserine Sulfhydrylase (Ägren et al, 2008). The metabolic pathways of biosynthesis of cysteine are important for the synthesis of de novo proteins and for reduced thiol as a component of the oxidative defense mechanisms. That occurs in the dormant state of ''Mycobacterium tuberculosis''.
-The cysteine synthase CysM is a O-phosphoserine-specific cysteine synthase which belongs to the fold type II pyridoxal 5’-phosphate-dependant enzymes (Ägren et al, 2008). The side chain of Arg220 interacts with the phosphate group by an hydrogen bound allowing the O-phosphoserine bound to the enzyme.
+The cysteine synthase CysM is a ''O''-phosphoserine-specific cysteine synthase which belongs to the fold type II pyridoxal 5’-phosphate-dependant enzymes (Ägren et al, 2008). The side chain of Arg220 interacts with the phosphate group by an hydrogen bound allowing the ''O''-phosphoserine bound to the enzyme.
-Thus, the signaling pathway of the cysteine synthase (CysM) of Mycobacterium tuberculosis leads an O-Phosphoserine binding with the enzyme O-Phosphoserine Sulfhydrylase. This mechanism is independent from the O-acetylserine and sulfate reduction pathway.
+Thus, the signaling pathway of the cysteine synthase (CysM) of ''Mycobacterium tuberculosis'' leads an ''O''-Phosphoserine binding with the enzyme ''O''-Phosphoserine Sulfhydrylase. This mechanism is independent from the ''O''-acetylserine and sulfate reduction pathway.
-According to researches on biosynthetic pathway in Mycobacterium tuberculosis (Burns et al, 2008), there are three pathways implicated cysteine in this disease: the sulfide dependent pathway, the cystathionine pathway and the CysO-thiocarboxylate pathway. For the CysO depending pathway, transcriptional profile analysis shown that cysM and cysO are upregulated under oxidative stress conditions. Moreover, the thiocarboxylate are much more resistant to oxidation than thiols. Thus, when the disease occurs, the environment becomes highly oxidizing due to the macrophages, leads to the cysteine biosynthesis. The CysO-thiocarboxylate evolves as an oxidation resistant form of sulfide, thiol is favored for the cysteine biosynthesis.
+According to researches on biosynthetic pathway in ''Mycobacterium tuberculosis'' (Burns et al, 2008), there are three pathways implicated cysteine in this disease: the sulfide dependent pathway, the cystathionine pathway and the CysO-thiocarboxylate pathway. For the CysO depending pathway, transcriptional profile analysis shown that cysM and cysO are upregulated under oxidative stress conditions. Moreover, the thiocarboxylate are much more resistant to oxidation than thiols. Thus, when the disease occurs, the environment becomes highly oxidizing due to the macrophages, leads to the cysteine biosynthesis. The CysO-thiocarboxylate evolves as an oxidation resistant form of sulfide, thiol is favored for the cysteine biosynthesis.