Sandbox Reserved 1501: Difference between revisions
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<StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> | ||
Encoded in the ''Saccharomyces cerevisiae'' (strain ATCC 204508 / S288c) gene ''CYB2'' the protein Flavocytochrome b(2) is an | Encoded in the ''Saccharomyces cerevisiae'' (strain ATCC 204508 / S288c) gene ''CYB2'' the protein Flavocytochrome b(2) is an oxidoreductase categorized according to IUB as EC: 1.1.2.3. | ||
==Function== | ==Function== | ||
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==Reaction== | ==Reaction== | ||
Flavocytochrome b(2) catalyzes dehydrogenation of L-lactate and the coupled cytochrome c reduction. | |||
Similar oxidants that can be used to perform the reaction ''in vitro'' are ferricyanide, phenazine methosulfate and quinone (experiment first performed 1963 by Nygaard<ref>PMID: 14480786</ref>, later in 1966 described by Symons and Burgoyne<ref>https://doi.org/10.1016/0076-6879(66)09064-5</ref>). | Similar oxidants that can be used to perform the reaction ''in vitro'' are ferricyanide, phenazine methosulfate and quinone (experiment first performed 1963 by Nygaard<ref>PMID: 14480786</ref>, later in 1966 described by Symons and Burgoyne<ref>https://doi.org/10.1016/0076-6879(66)09064-5</ref>). | ||
The Yeasts L-Lactate Dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids (Nygaard 1963<ref>PMID: 14480786</ref>). | The Yeasts L-Lactate Dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids (Nygaard 1963<ref>PMID: 14480786</ref>). | ||
The Enzyme shows a specificity for L-lactate but none for the D-isomer or -hydroxybutyrate. | The Enzyme shows a specificity for L-lactate but none for the D-isomer or -hydroxybutyrate. | ||
[[Image:Reaction1.JPG|center|x150px]] | |||
==Structure highlight== | ==Structure highlight== | ||
<!-- Global Symmetry Cyclic - C4--> | <!-- Global Symmetry Cyclic - C4--> | ||
