Sandbox Reserved 1501: Difference between revisions
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Flavocytochrome b(2) catalyzes dehydrogenation of L-lactate and the coupled cytochrome c reduction. | Flavocytochrome b(2) catalyzes dehydrogenation of L-lactate and the coupled cytochrome c reduction. | ||
Similar oxidants that can be used to perform the reaction ''in vitro'' are ferricyanide, phenazine methosulfate and quinone (experiment first performed 1963 by Nygaard<ref>PMID: 14480786</ref>, later in 1966 described by Symons and Burgoyne<ref>10.1016/0076-6879(66)09064-5</ref>). | Similar oxidants that can be used to perform the reaction ''in vitro'' are ferricyanide, phenazine methosulfate and quinone (experiment first performed 1963 by Nygaard<ref>PMID: 14480786</ref>, later in 1966 described by Symons and Burgoyne<ref>DOI:10.1016/0076-6879(66)09064-5</ref>). | ||
The yeasts L-lactate dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids (Nygaard, 1963<ref>PMID: 14480786</ref>). | The yeasts L-lactate dehydrogenase can be inhibited by heavy metals, oxygen, glycerate, oxalate, malate, phenylpyruvate and fatty acids (Nygaard, 1963<ref>PMID: 14480786</ref>). | ||
The enzyme shows a specificity for L-lactate but none for the D-isomer or -hydroxybutyrate. | The enzyme shows a specificity for L-lactate but none for the D-isomer or -hydroxybutyrate. | ||
