6qaz: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6qaz is ~~ON HOLD until Paper~~ Publication

==Crystal structure of gp41-1 intein==

== Structural highlights ==

<table><tr><td colspan='2'>[[6qaz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QAZ FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qaz OCA], [http://pdbe.org/6qaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qaz RCSB], [http://www.ebi.ac.uk/pdbsum/6qaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qaz ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 A-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein (HINT) superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins.

~~Authors~~:

The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.,Michael Beyer H, Malgorzata Mikula K, Li M, Wlodawer A, Iwai H FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. PMID:31665813<ref>PMID:31665813</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Large Structures]]

[[Category: Beyer, H M]]

[[Category: Iwai, H]]

[[Category: Li, M]]

[[Category: Mikula, K M]]

[[Category: Wlodawer, A]]

[[Category: Intein]]

[[Category: Metagenomic]]

[[Category: Protein-splicing]]

[[Category: Unknown function]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6qaz is ON HOLD  until Paper Publication
+==Crystal structure of gp41-1 intein==
+<StructureSection load='6qaz' size='340' side='right'caption='[[6qaz]], [[Resolution|resolution]] 1.02&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6qaz]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QAZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QAZ FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qaz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qaz OCA], [http://pdbe.org/6qaz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qaz RCSB], [http://www.ebi.ac.uk/pdbsum/6qaz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qaz ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 A-resolution crystal structure of a fused variant from the naturally split gp41-1 intein, previously identified from environmental metagenomic sequence data. The structure of the 125-residue gp41-1 intein revealed a compact pseudo-C2-symmetry commonly found in the Hedgehog/Intein (HINT) superfamily with extensive charge-charge interactions between the split N- and C-terminal intein fragments that are common among naturally occurring split inteins. We successfully created orthogonal split inteins by engineering a similar charge network into the same region of a cis-splicing intein. This strategy could be applicable for creating novel natural-like split inteins from other, more prevalent cis-splicing inteins.
-Authors:
+The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.,Michael Beyer H, Malgorzata Mikula K, Li M, Wlodawer A, Iwai H FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. PMID:31665813<ref>PMID:31665813</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6qaz" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Beyer, H M]]
+[[Category: Iwai, H]]
+[[Category: Li, M]]
+[[Category: Mikula, K M]]
+[[Category: Wlodawer, A]]
+[[Category: Intein]]
+[[Category: Metagenomic]]
+[[Category: Protein-splicing]]
+[[Category: Unknown function]]