2qze: Difference between revisions
New page: left|200px {{Structure |PDB= 2qze |SIZE=350|CAPTION= <scene name='initialview01'>2qze</scene>, resolution 2.90Å |SITE= |LIGAND= |ACTIVITY= |GENE= |DOMAIN= |R... |
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{{STRUCTURE_2qze| PDB=2qze | SCENE= }} | |||
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'''Monoclinic Mimivirus Capping Enzyme Triphosphatase.''' | '''Monoclinic Mimivirus Capping Enzyme Triphosphatase.''' | ||
==Overview== | |||
The RNA triphosphatase (RTPase) components of the mRNA capping apparatus are a bellwether of eukaryal taxonomy. Fungal and protozoal RTPases belong to the triphosphate tunnel metalloenzyme (TTM) family, exemplified by yeast Cet1. Several large DNA viruses encode metal-dependent RTPases unrelated to the cysteinyl-phosphatase RTPases of their metazoan host organisms. The origins of DNA virus RTPases are unclear because they are structurally uncharacterized. Mimivirus, a giant virus of amoeba, resembles poxviruses in having a trifunctional capping enzyme composed of a metal-dependent RTPase module fused to guanylyltransferase (GTase) and guanine-N7 methyltransferase domains. The crystal structure of mimivirus RTPase reveals a minimized tunnel fold and an active site strikingly similar to that of Cet1. Unlike homodimeric fungal RTPases, mimivirus RTPase is a monomer. The mimivirus TTM-type RTPase-GTase fusion resembles the capping enzymes of amoebae, providing evidence that the ancestral large DNA virus acquired its capping enzyme from a unicellular host. | |||
==About this Structure== | ==About this Structure== | ||
2QZE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mimivirus Mimivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZE OCA]. | 2QZE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mimivirus Mimivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZE OCA]. | ||
==Reference== | |||
Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domain., Benarroch D, Smith P, Shuman S, Structure. 2008 Apr;16(4):501-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18400173 18400173] | |||
[[Category: Mimivirus]] | [[Category: Mimivirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 25: | ||
[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
[[Category: Smith, P.]] | [[Category: Smith, P.]] | ||
[[Category: | [[Category: Capping]] | ||
[[Category: | [[Category: Hydrolase]] | ||
[[Category: | [[Category: Mimivirus]] | ||
[[Category: | [[Category: Monoclinic]] | ||
[[Category: | [[Category: Mrna capping]] | ||
[[Category: | [[Category: Mrna processing]] | ||
[[Category: | [[Category: Multifunctional enzyme]] | ||
[[Category: | [[Category: Nucleotidyltransferase]] | ||
[[Category: | [[Category: S-adenosyl-l-methionine]] | ||
[[Category: | [[Category: Transferase]] | ||
[[Category: | [[Category: Triphosphatase]] | ||
[[Category: | [[Category: Tunnel]] | ||
[[Category: | [[Category: Viral protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:27:58 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 06:27, 24 April 2008
Monoclinic Mimivirus Capping Enzyme Triphosphatase.
Overview
The RNA triphosphatase (RTPase) components of the mRNA capping apparatus are a bellwether of eukaryal taxonomy. Fungal and protozoal RTPases belong to the triphosphate tunnel metalloenzyme (TTM) family, exemplified by yeast Cet1. Several large DNA viruses encode metal-dependent RTPases unrelated to the cysteinyl-phosphatase RTPases of their metazoan host organisms. The origins of DNA virus RTPases are unclear because they are structurally uncharacterized. Mimivirus, a giant virus of amoeba, resembles poxviruses in having a trifunctional capping enzyme composed of a metal-dependent RTPase module fused to guanylyltransferase (GTase) and guanine-N7 methyltransferase domains. The crystal structure of mimivirus RTPase reveals a minimized tunnel fold and an active site strikingly similar to that of Cet1. Unlike homodimeric fungal RTPases, mimivirus RTPase is a monomer. The mimivirus TTM-type RTPase-GTase fusion resembles the capping enzymes of amoebae, providing evidence that the ancestral large DNA virus acquired its capping enzyme from a unicellular host.
About this Structure
2QZE is a Single protein structure of sequence from Mimivirus. Full crystallographic information is available from OCA.
Reference
Characterization of a trifunctional mimivirus mRNA capping enzyme and crystal structure of the RNA triphosphatase domain., Benarroch D, Smith P, Shuman S, Structure. 2008 Apr;16(4):501-12. PMID:18400173 Page seeded by OCA on Thu Apr 24 09:27:58 2008
