6nx4: Difference between revisions
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The | ==Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase (eEF-2K)== | ||
<StructureSection load='6nx4' size='340' side='right'caption='[[6nx4]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6nx4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NX4 FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/[Elongation_factor_2]_kinase [Elongation factor 2] kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.20 2.7.11.20] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nx4 OCA], [http://pdbe.org/6nx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6nx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nx4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/EF2K_HUMAN EF2K_HUMAN]] Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.<ref>PMID:14709557</ref> <ref>PMID:9144159</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Eukaryotic elongation factor 2 kinase (eEF-2K), an atypical calmodulin-activated protein kinase, regulates translational elongation by phosphorylating its substrate, eukaryotic elongation factor 2 (eEF-2), thereby reducing its affinity for the ribosome. The activation and activity of eEF-2K are critical for survival under energy-deprived conditions and is implicated in a variety of essential physiological processes. Previous biochemical experiments have indicated that the binding site for the substrate eEF-2 is located in the C-terminal domain of eEF-2K, a region predicted to harbor several alpha-helical repeats. Here, using NMR methodology we have determined the solution structure of a C-terminal fragment of eEF-2K, eEF-2K562-725 that encodes two alpha-helical repeats. The structure of eEF-2K562-725 shows signatures characteristic of TPR domains and of their SEL1-like sub-family. Further, using the analyses of NMR spectral perturbations and ITC measurements, we have localized the eEF-2 binding site on eEF-2K562-725. We find that eEF-2K562-725 engages eEF-2 with an affinity comparable to that of the full-length enzyme. Further, eEF-2K562-725 is able to inhibit the phosphorylation of eEF-2 by full-length eEF-2K in trans. Our present studies establish that eEF-2K562-725 encodes the major elements necessary to enable the eEF-2K/eEF-2 interactions. | |||
Solution Structure of the Carboxy-Terminal Tandem Repeat Domain of Eukaryotic Elongation Factor 2 Kinase and its Role in Substrate Recognition.,Piserchio A, Will N, Giles DH, Hajredini F, Dalby KN, Ghose R J Mol Biol. 2019 May 17. pii: S0022-2836(19)30290-6. doi:, 10.1016/j.jmb.2019.05.019. PMID:31108082<ref>PMID:31108082</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6nx4" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Dalby, K N]] | |||
[[Category: Ghose, R]] | |||
[[Category: Giles, D H]] | |||
[[Category: Hajredini, F]] | |||
[[Category: Piserchio, A]] | [[Category: Piserchio, A]] | ||
[[Category: Will, N]] | [[Category: Will, N]] | ||
[[Category: | [[Category: Binding domain]] | ||
[[Category: | [[Category: Eef2]] | ||
[[Category: Eef2k]] | |||
[[Category: Elongation]] | |||
[[Category: Kinase]] | |||
[[Category: Sel1]] | |||
[[Category: Tpr]] | |||
[[Category: Transferase]] | |||
[[Category: Translation]] | |||
