3brd: Difference between revisions

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New page: left|200px {{Structure |PDB= 3brd |SIZE=350|CAPTION= <scene name='initialview01'>3brd</scene>, resolution 2.210Å |SITE= <scene name='pdbsite=AC1:Edo+Binding+Site...
 
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[[Image:3brd.jpg|left|200px]]
[[Image:3brd.jpg|left|200px]]


{{Structure
<!--
|PDB= 3brd |SIZE=350|CAPTION= <scene name='initialview01'>3brd</scene>, resolution 2.210&Aring;
The line below this paragraph, containing "STRUCTURE_3brd", creates the "Structure Box" on the page.
|SITE= <scene name='pdbsite=AC1:Edo+Binding+Site+For+Residue+A+1'>AC1</scene>, <scene name='pdbsite=AC2:Edo+Binding+Site+For+Residue+A+2'>AC2</scene>, <scene name='pdbsite=AC3:Edo+Binding+Site+For+Residue+A+3'>AC3</scene>, <scene name='pdbsite=AC4:Edo+Binding+Site+For+Residue+A+4'>AC4</scene>, <scene name='pdbsite=AC5:Edo+Binding+Site+For+Residue+A+5'>AC5</scene>, <scene name='pdbsite=AC6:Edo+Binding+Site+For+Residue+D+6'>AC6</scene>, <scene name='pdbsite=AC7:Edo+Binding+Site+For+Residue+C+16'>AC7</scene>, <scene name='pdbsite=AC8:Edo+Binding+Site+For+Residue+A+8'>AC8</scene>, <scene name='pdbsite=AC9:Edo+Binding+Site+For+Residue+A+9'>AC9</scene> and <scene name='pdbsite=BC1:Edo+Binding+Site+For+Residue+A+10'>BC1</scene>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
|ACTIVITY=
or leave the SCENE parameter empty for the default display.
|GENE= lag-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]), lin-12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans])
-->
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd01176 IPT_RBP-Jkappa], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam09271 LAG1-DNAbind], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam09270 Beta-trefoil]</span>
{{STRUCTURE_3brd| PDB=3brd  | SCENE= }}  
|RELATEDENTRY=[[3brf|3BRF]], [[3brg|3BRG]]
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3brd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3brd OCA], [http://www.ebi.ac.uk/pdbsum/3brd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3brd RCSB]</span>
}}


'''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121'''
'''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121'''


==Overview==
The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.


==About this Structure==
==About this Structure==
3BRD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRD OCA].  
3BRD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRD OCA].  
==Reference==
RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18381292 18381292]
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Kovall, R A.]]
[[Category: Kovall, R A.]]
[[Category: Wilson, J J.]]
[[Category: Wilson, J J.]]
[[Category: ank repeat]]
[[Category: Ank repeat]]
[[Category: developmental protein]]
[[Category: Developmental protein]]
[[Category: differentiation]]
[[Category: Differentiation]]
[[Category: dna binding protein/dna complex]]
[[Category: Dna binding protein/dna complex]]
[[Category: dna-binding]]
[[Category: Dna-binding]]
[[Category: egf-like domain]]
[[Category: Egf-like domain]]
[[Category: glycoprotein]]
[[Category: Glycoprotein]]
[[Category: membrane]]
[[Category: Membrane]]
[[Category: notch]]
[[Category: Notch]]
[[Category: protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: signaling]]
[[Category: Signaling]]
[[Category: transcription]]
[[Category: Transcription]]
[[Category: transmembrane]]
[[Category: Transmembrane]]
 
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 2 11:59:28 2008''
Retrieved from "https://proteopedia.org/3brd"