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'''Unreleased structure'''


The entry 6qq5 is ON HOLD
==Cryo-EM structure of dimeric quinol dependent nitric oxide reductase (qNOR) from Alcaligenes xylosoxidans==
<StructureSection load='6qq5' size='340' side='right'caption='[[6qq5]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qq5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_xylosoxidans"_yabuuchi_and_ohyama_1971 "achromobacter xylosoxidans" yabuuchi and ohyama 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QQ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QQ5 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">norB_1, ERS451415_02175 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85698 "Achromobacter xylosoxidans" Yabuuchi and Ohyama 1971])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_reductase_(cytochrome_c) Nitric-oxide reductase (cytochrome c)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.5 1.7.2.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qq5 OCA], [http://pdbe.org/6qq5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qq5 RCSB], [http://www.ebi.ac.uk/pdbsum/6qq5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qq5 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Quinol-dependent nitric oxide reductases (qNORs) are membrane-integrated, iron-containing enzymes of the denitrification pathway, which catalyze the reduction of nitric oxide (NO) to the major ozone destroying gas nitrous oxide (N2O). Cryo-electron microscopy structures of active qNOR from Alcaligenes xylosoxidans and an activity-enhancing mutant have been determined to be at local resolutions of 3.7 and 3.2 A, respectively. They unexpectedly reveal a dimeric conformation (also confirmed for qNOR from Neisseria meningitidis) and define the active-site configuration, with a clear water channel from the cytoplasm. Structure-based mutagenesis has identified key residues involved in proton transport and substrate delivery to the active site of qNORs. The proton supply direction differs from cytochrome c-dependent NOR (cNOR), where water molecules from the cytoplasm serve as a proton source similar to those from cytochrome c oxidase.


Authors: Gopalasingam, C.C., Johnson, R.M., Chiduza, G.N., Tosha, T., Yamamoto, M., Shiro, Y., Antonyuk, S.V., Muench, S.P., Hasnain, S.S.
Dimeric structures of quinol-dependent nitric oxide reductases (qNORs) revealed by cryo-electron microscopy.,Gopalasingam CC, Johnson RM, Chiduza GN, Tosha T, Yamamoto M, Shiro Y, Antonyuk SV, Muench SP, Hasnain SS Sci Adv. 2019 Aug 28;5(8):eaax1803. doi: 10.1126/sciadv.aax1803. eCollection 2019, Aug. PMID:31489376<ref>PMID:31489376</ref>


Description: Cryo-EM structure of dimeric quinol dependent nitric oxide reductase (qNOR) from Alcaligenes xylosoxidans
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6qq5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Achromobacter xylosoxidans yabuuchi and ohyama 1971]]
[[Category: Large Structures]]
[[Category: Antonyuk, S V]]
[[Category: Chiduza, G N]]
[[Category: Gopalasingam, C C]]
[[Category: Hasnain, S S]]
[[Category: Johnson, R M]]
[[Category: Muench, S P]]
[[Category: Shiro, Y]]
[[Category: Shiro, Y]]
[[Category: Gopalasingam, C.C]]
[[Category: Tosha, T]]
[[Category: Yamamoto, M]]
[[Category: Yamamoto, M]]
[[Category: Hasnain, S.S]]
[[Category: Homodimer]]
[[Category: Tosha, T]]
[[Category: Membrane protein]]
[[Category: Muench, S.P]]
[[Category: Oxidoreductase]]
[[Category: Antonyuk, S.V]]
[[Category: Proton transfer]]
[[Category: Johnson, R.M]]
[[Category: Chiduza, G.N]]
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