6qsp: Difference between revisions
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==Ketosynthase (ApeO) in Complex with its Chain Length Factor (ApeC) from Xenorhabdus doucetiae== | |||
<StructureSection load='6qsp' size='340' side='right'caption='[[6qsp]], [[Resolution|resolution]] 1.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6qsp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QSP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2iwz|2iwz]], [[1tqy|1tqy]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-ketoacyl-[acyl-carrier-protein]_synthase_I Beta-ketoacyl-[acyl-carrier-protein] synthase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsp OCA], [http://pdbe.org/6qsp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qsp RCSB], [http://www.ebi.ac.uk/pdbsum/6qsp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aryl polyene (APE) pigments are a widely distributed class of bacterial polyketides. So far, little is known about the biosynthesis of these compounds, which are produced by a novel type II polyketide synthase (PKS). We have identi-fied all enzymes involved in APE biosynthesis and determined their peculiar functions. The biosynthesis was recon-stituted in vitro and ACP-bound intermediates were assigned for each reaction step by HPLC-MS. Native mass spec-trometry experiments identified four stable complexes: the acyl-carrier proteins ApeE and ApeF bound to the thi-oesterase ApeK, the dehydratases ApeI and ApeP as well as the ketosynthase ApeO in complex with its chain-length factor ApeC. X-ray structures of the heterodimeric ApeO:ApeC and ApeI:ApeP complexes depict striking protein-protein interactions. Altogether, our study elucidated mechanistic aspects of APE biosynthesis that unifies elements of type II fatty acid and PKS systems, but in addition includes novel enzyme complexes. | |||
An Uncommon Type II PKS Catalyzes Biosynthesis of Aryl Polyene Pigments.,Grammbitter GLC, Schmalhofer M, Karimi K, Schoner TA, Tobias NJ, Morgner N, Groll M, Bode HB J Am Chem Soc. 2019 Mar 25. doi: 10.1021/jacs.8b10776. PMID:30908039<ref>PMID:30908039</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6qsp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Bode, H]] | |||
[[Category: Grammbitter, G L.C]] | |||
[[Category: Groll, M]] | |||
[[Category: Schmalhofer, M]] | |||
[[Category: Aryl polyene pigment]] | |||
[[Category: Biosynthesis]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Chain elongation]] | |||
[[Category: Claisen condensation]] | |||
[[Category: Ketoacyl synthase]] | |||
[[Category: Type ii polyketide synthase]] | |||
