Human Keto Acyl Reductase: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 11: | Line 11: | ||
== Structural highlights == Crystal structure of human mitochondrial heteroterameric KAR is solved at 2.34 Å. The co-factors NAD is selectively bound to the HSD17B8 subunits whereas NADP is bound to the CBR4 subunits. Both NAD and NADP are stabilized by several conserved hydrogen bonding interactions in their respective binding sites. The Asp42, Arg12 and Arg 34 are the crucial residues for co-factor recognition. Acetate ion is also bound near NAD, which identifies the fatty acyl chain binding pocket. Ser 135, Tyr148 and Lys152 in CBR4 subunit are the catalytic triad residues involved in the catalysis. <ref>PMID:25203508</ref> | == Structural highlights == Crystal structure of human mitochondrial heteroterameric KAR is solved at 2.34 Å. The co-factors NAD is selectively bound to the HSD17B8 subunits whereas NADP is bound to the CBR4 subunits. Both NAD and NADP are stabilized by several conserved hydrogen bonding interactions in their respective binding sites. The Asp42, Arg12 and Arg 34 are the crucial residues for co-factor recognition. Acetate ion is also bound near NAD, which identifies the fatty acyl chain binding pocket. Ser 135, Tyr148 and Lys152 in CBR4 subunit are the catalytic triad residues involved in the catalysis. <ref>PMID:25203508</ref> | ||
<scene name='80/809823/All_nadp/2'>NADP</scene> is bound in each of the four subunits in heterotetrameric structure. <scene name='80/809823/Glycerol/1'>glycerol</scene> is also bound in the structure. | <scene name='80/809823/All_nadp/2'>NADP</scene> is bound in each of the four subunits in heterotetrameric structure. <scene name='80/809823/Glycerol/1'>glycerol</scene> is also bound in the structure. <scene name='80/809823/Nad-a/1'>NAD</scene>is bound to the HSD17B8 subunit | ||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
