4cyg: Difference between revisions

Revision as of 07:38, 14 September 2022

The structure of vanin-1: defining the link between metabolic disease, oxidative stress and inflammation

Structural highlights

4cyg is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NAG, PEG, RRV
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VNN1_HUMAN] Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.^[1] ^[2]

Publication Abstract from PubMed

Although part of the coenzyme A pathway, vanin 1 (also known as pantetheinase) sits on the cell surface of many cell types as an ectoenzyme, catalyzing the breakdown of pantetheine to pantothenic acid (vitamin B5) and cysteamine, a strong reducing agent. Vanin 1 was initially discovered as a protein involved in the homing of leukocytes to the thymus. Numerous studies have shown that vanin 1 is involved in inflammation, and more recent studies have shown a key role in metabolic disease. Here, the X-ray crystal structure of human vanin 1 at 2.25 A resolution is presented, which is the first reported structure from the vanin family, as well as a crystal structure of vanin 1 bound to a specific inhibitor. These structures illuminate how vanin 1 can mediate its biological roles by way of both enzymatic activity and protein-protein interactions. Furthermore, it sheds light on how the enzymatic activity is regulated by a novel allosteric mechanism at a domain interface.

The structure of vanin 1: a key enzyme linking metabolic disease and inflammation.,Boersma YL, Newman J, Adams TE, Cowieson N, Krippner G, Bozaoglu K, Peat TS Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3320-9. doi:, 10.1107/S1399004714022767. Epub 2014 Nov 28. PMID:25478849^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maras B, Barra D, Dupre S, Pitari G. Is pantetheinase the actual identity of mouse and human vanin-1 proteins? FEBS Lett. 1999 Nov 19;461(3):149-52. PMID:10567687
↑ Martin F, Malergue F, Pitari G, Philippe JM, Philips S, Chabret C, Granjeaud S, Mattei MG, Mungall AJ, Naquet P, Galland F. Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes. Immunogenetics. 2001 May-Jun;53(4):296-306. PMID:11491533
↑ Boersma YL, Newman J, Adams TE, Cowieson N, Krippner G, Bozaoglu K, Peat TS. The structure of vanin 1: a key enzyme linking metabolic disease and inflammation. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3320-9. doi:, 10.1107/S1399004714022767. Epub 2014 Nov 28. PMID:25478849 doi:https://dx.doi.org/10.1107/S1399004714022767

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Maras B, Barra D, Dupre S, Pitari G. Is pantetheinase the actual identity of mouse and human vanin-1 proteins? FEBS Lett. 1999 Nov 19;461(3):149-52. PMID:10567687

[2] Martin F, Malergue F, Pitari G, Philippe JM, Philips S, Chabret C, Granjeaud S, Mattei MG, Mungall AJ, Naquet P, Galland F. Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes. Immunogenetics. 2001 May-Jun;53(4):296-306. PMID:11491533

[3] Boersma YL, Newman J, Adams TE, Cowieson N, Krippner G, Bozaoglu K, Peat TS. The structure of vanin 1: a key enzyme linking metabolic disease and inflammation. Acta Crystallogr D Biol Crystallogr. 2014 Dec 1;70(Pt 12):3320-9. doi:, 10.1107/S1399004714022767. Epub 2014 Nov 28. PMID:25478849 doi:https://dx.doi.org/10.1107/S1399004714022767

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='4cyg' size='340' side='right'caption='[[4cyg]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4cyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CYG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CYG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4cyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CYG FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=RRV:(2R)-2,4-DIHYDROXY-N-[(3S)-3-HYDROXY-4-PHENYLBUTYL]-3,3-DIMETHYLBUTANAMIDE'>RRV</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=RRV:(2R)-2,4-DIHYDROXY-N-[(3S)-3-HYDROXY-4-PHENYLBUTYL]-3,3-DIMETHYLBUTANAMIDE'>RRV</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cyf|4cyf]], [[4cyu|4cyu]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cyg OCA], [https://pdbe.org/4cyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cyg RCSB], [https://www.ebi.ac.uk/pdbsum/4cyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cyg ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pantetheine_hydrolase Pantetheine hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.92 3.5.1.92] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4cyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cyg OCA], [http://pdbe.org/4cyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cyg RCSB], [http://www.ebi.ac.uk/pdbsum/4cyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cyg ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VNN1_HUMAN VNN1_HUMAN]] Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.<ref>PMID:10567687</ref> <ref>PMID:11491533</ref>
+[[https://www.uniprot.org/uniprot/VNN1_HUMAN VNN1_HUMAN]] Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.<ref>PMID:10567687</ref> <ref>PMID:11491533</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Pantetheine hydrolase]]
+[[Category: Adams TE]]
-[[Category: Adams, T E]]
+[[Category: Boersma YL]]
-[[Category: Boersma, Y L]]
+[[Category: Bozaoglu K]]
-[[Category: Bozaoglu, K]]
+[[Category: Cowieson N]]
-[[Category: Cowieson, N]]
+[[Category: Krippner G]]
-[[Category: Krippner, G]]
+[[Category: Lucent D]]
-[[Category: Lucent, D]]
+[[Category: Newman J]]
-[[Category: Newman, J]]
+[[Category: Peat TS]]
-[[Category: Peat, T S]]
+[[Category: Sparrow L]]
-[[Category: Sparrow, L]]
-[[Category: Coa biosynthesis]]
-[[Category: Colitis]]
-[[Category: Hydrolase]]
-[[Category: Inflammation]]
-[[Category: Oxidative stress]]

4cyg: Difference between revisions

Revision as of 07:38, 14 September 2022

The structure of vanin-1: defining the link between metabolic disease, oxidative stress and inflammation

Structural highlights

Function

Publication Abstract from PubMed

References

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