6jcf: Difference between revisions
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==Cryogenic structure of HIV-1 Integrase catalytic core domain by synchrotron== | |||
<StructureSection load='6jcf' size='340' side='right'caption='[[6jcf]], [[Resolution|resolution]] 2.15Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6jcf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JCF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JCF FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jcf OCA], [http://pdbe.org/6jcf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jcf RCSB], [http://www.ebi.ac.uk/pdbsum/6jcf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jcf ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
HIV-1 integrase (HIV-1 IN) is an enzyme produced by the HIV-1 virus that integrates genetic material of the virus into the DNA of infected human cells. HIV-1 IN acts as a key component of the Retroviral Pre-Integration Complex (PIC). Protein dynamics could play an important role during the catalysis of HIV-1 IN; however, this process has not yet been fully elucidated. X-ray free electron laser (XFEL) together with nuclear magnetic resonance (NMR) could provide information regarding the dynamics during this catalysis reaction. Here, we report the non-cryogenic crystal structure of HIV-1 IN catalytic core domain at 2.5 A using microcrystals in XFELs. Compared to the cryogenic structure at 2.1 A using conventional synchrotron crystallography, there was a good agreement between the two structures, except for a catalytic triad formed by Asp64, Asp116, and Glu152 (DDE) and the lens epithelium-derived growth factor binding sites. The helix III region of the 140-153 residues near the active site and the DDE triad show a higher dynamic profile in the non-cryogenic structure, which is comparable to dynamics data obtained from NMR spectroscopy in solution state. | |||
Non-Cryogenic Structure and Dynamics of HIV-1 Integrase Catalytic Core Domain by X-ray Free-Electron Lasers.,Park JH, Yun JH, Shi Y, Han J, Li X, Jin Z, Kim T, Park J, Park S, Liu H, Lee W Int J Mol Sci. 2019 Apr 20;20(8). pii: ijms20081943. doi: 10.3390/ijms20081943. PMID:31010024<ref>PMID:31010024</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6jcf" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Han, J]] | |||
[[Category: Kim, T H]] | |||
[[Category: Lee, W]] | |||
[[Category: Park, J H]] | |||
[[Category: Yun, J H]] | |||
[[Category: Cryogenic]] | |||
[[Category: Hiv]] | |||
[[Category: Hiv-1]] | |||
[[Category: Integrase]] | |||
[[Category: Viral protein]] | |||
Revision as of 10:40, 17 July 2019
Cryogenic structure of HIV-1 Integrase catalytic core domain by synchrotron
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