6r3c: Difference between revisions
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The | ==Solution structure of birch pollen allergen Bet v 1a== | ||
<StructureSection load='6r3c' size='340' side='right'caption='[[6r3c]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6r3c]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R3C FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r3c OCA], [http://pdbe.org/6r3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r3c RCSB], [http://www.ebi.ac.uk/pdbsum/6r3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r3c ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/BEV1A_BETPN BEV1A_BETPN]] May be a general steroid carrier protein (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hazelnut is one of the most frequent causes of food allergy. The major hazel allergen in Northern Europe is Cor a 1, which is homologous to the major birch pollen allergen Bet v 1. Both allergens belong to the pathogenesis related class PR-10. We determined the solution structure of Cor a 1.0401 from hazelnut and identified a natural ligand of the protein. The structure reveals the protein fold characteristic for PR-10 family members, which consists of a seven-stranded antiparallel beta-sheet, two short alpha-helices arranged in V-shape and a long C-terminal alpha-helix encompassing a hydrophobic pocket. However, despite the structural similarities between Cor a 1 and Bet v 1, they bind different ligands. We have shown previously that Bet v 1 binds to quercetin-3-O-sophoroside. Here, we isolated Cor a 1 from hazel pollen and identified the bound ligand, quercetin-3-O-(2"-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside, by mass spectrometry and nuclear magnetic resonance spectroscopy (NMR). NMR experiments were performed to confirm binding. Remarkably, although it has been shown that PR-10 allergens show promiscuous binding behaviour in vitro, we can demonstrate that Cor a 1.0401 and Bet v 1.0101 exhibit highly selective binding for their specific ligand but not for the respective ligand of the other allergen. | |||
Identification of a natural ligand of the hazel allergen Cor a 1.,Jacob T, von Loetzen CS, Reuter A, Lacher U, Schiller D, Schobert R, Mahler V, Vieths S, Rosch P, Schweimer K, Wohrl BM Sci Rep. 2019 Jun 18;9(1):8714. doi: 10.1038/s41598-019-44999-2. PMID:31213622<ref>PMID:31213622</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6r3c" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Schweimer, K]] | [[Category: Schweimer, K]] | ||
[[Category: Allergen]] | |||
[[Category: Bet v 1]] | |||
[[Category: Birch pollen allergen]] | |||
Latest revision as of 06:02, 3 July 2019
Solution structure of birch pollen allergen Bet v 1a
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