4gyc: Difference between revisions

Revision as of 07:25, 26 October 2022

Structure of the SRII(D75N mutant)/HtrII Complex in I212121 space group ("U" shape)

Structural highlights

4gyc is a 2 chain structure with sequence from Natronomonas pharaonis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	LFA, RET
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BACS2_NATPH Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.

Publication Abstract from PubMed

The complex of sensory rhodopsin II (NpSRII) with its cognate transducer (NpHtrII) mediates negative phototaxis in halobacteria Natronomonas pharaonis. Upon light activation NpSRII triggers, by means of NpHtrII, a signal transduction chain homologous to the two component system in eubacterial chemotaxis. Here we report on the crystal structure of the ground state of the mutant NpSRII-D75N/NpHtrII complex in the space group I212121. Mutations of this aspartic acid in light-driven proton pumps dramatically modify or/and inhibit protein functions. However, in vivo studies show that the similar D75N mutation retains functionality of the NpSRII/NpHtrII complex. The structure provides the molecular basis for the explanation of the unexpected observation that the wild and the mutant complexes display identical physiological response on light excitation.

Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer.,Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Balandin T, Remeeva A, Engelhard M, Buldt G, Gordeliy V J Photochem Photobiol B. 2013 Jun 5;123:55-8. doi:, 10.1016/j.jphotobiol.2013.03.008. Epub 2013 Mar 29. PMID:23619282^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Balandin T, Remeeva A, Engelhard M, Buldt G, Gordeliy V. Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer. J Photochem Photobiol B. 2013 Jun 5;123:55-8. doi:, 10.1016/j.jphotobiol.2013.03.008. Epub 2013 Mar 29. PMID:23619282 doi:https://dx.doi.org/10.1016/j.jphotobiol.2013.03.008

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OCA

[1] Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Balandin T, Remeeva A, Engelhard M, Buldt G, Gordeliy V. Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer. J Photochem Photobiol B. 2013 Jun 5;123:55-8. doi:, 10.1016/j.jphotobiol.2013.03.008. Epub 2013 Mar 29. PMID:23619282 doi:https://dx.doi.org/10.1016/j.jphotobiol.2013.03.008

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='4gyc' size='340' side='right'caption='[[4gyc]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4gyc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35678 Atcc 35678]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GYC FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4gyc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GYC FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1h2s|1h2s]], [[4gy6|4gy6]], [[4gy8|4gy8]], [[4gya|4gya]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyc OCA], [https://pdbe.org/4gyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gyc RCSB], [https://www.ebi.ac.uk/pdbsum/4gyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gyc ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sop2, sopII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678]), htr2, htrII ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2257 ATCC 35678])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gyc OCA], [http://pdbe.org/4gyc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gyc RCSB], [http://www.ebi.ac.uk/pdbsum/4gyc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gyc ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/BACS2_NATPH BACS2_NATPH]] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light. [[http://www.uniprot.org/uniprot/HTR2_NATPH HTR2_NATPH]] Transduces signals from the phototaxis receptor sensory rhodopsin II (SR-II) to the flagellar motor. Responds to light changes through the variation of the level of methylation. Also acts as a chemotransducer.
+[https://www.uniprot.org/uniprot/BACS2_NATPH BACS2_NATPH] Involved in the control of phototaxis. Seems to activate a methyl-accepting protein (HTR-II). Photoreceptor for blue light.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 20: @@
 ==See Also==
-*[[Bacteriorhodopsin|Bacteriorhodopsin]]
+*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
-*[[Rhodopsin|Rhodopsin]]
+*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Atcc 35678]]
 [[Category: Large Structures]]
-[[Category: Balandin, T]]
+[[Category: Natronomonas pharaonis]]
-[[Category: Borshchevskiy, V]]
+[[Category: Balandin T]]
-[[Category: Bueldt, G]]
+[[Category: Borshchevskiy V]]
-[[Category: Engelhard, M]]
+[[Category: Bueldt G]]
-[[Category: Gordeliy, V]]
+[[Category: Engelhard M]]
-[[Category: Grudinin, S]]
+[[Category: Gordeliy V]]
-[[Category: Gushchin, I]]
+[[Category: Grudinin S]]
-[[Category: Ishchenko, A]]
+[[Category: Gushchin I]]
-[[Category: Klare, J]]
+[[Category: Ishchenko A]]
-[[Category: Remeeva, A]]
+[[Category: Klare J]]
-[[Category: Round, E]]
+[[Category: Remeeva A]]
-[[Category: Utrobin, P]]
+[[Category: Round E]]
-[[Category: Membrane protein]]
+[[Category: Utrobin P]]
-[[Category: Photoreceptor]]

4gyc: Difference between revisions

Revision as of 07:25, 26 October 2022

Structure of the SRII(D75N mutant)/HtrII Complex in I212121 space group ("U" shape)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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