6r47: Difference between revisions
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The | ==The structure of pistol ribozyme bound to magnesium== | ||
<StructureSection load='6r47' size='340' side='right'caption='[[6r47]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6r47]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R47 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CBV:5-BROMOCYTIDINE+5-(DIHYDROGEN+PHOSPHATE)'>CBV</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r47 OCA], [http://pdbe.org/6r47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r47 RCSB], [http://www.ebi.ac.uk/pdbsum/6r47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r47 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Comparison of the secondary and three-dimensional structures of the hammerhead and pistol ribozymes reveals many close similarities, so in this work we have asked if they are mechanistically identical. We have determined a new crystal structure of the pistol ribozyme and have shown that G40 acts as general base in the cleavage reaction. The conformation in the active site ensures an in-line attack of the O2' nucleophile, and the conformation at the scissile phosphate and the position of the general base are closely similar to those in the hammerhead ribozyme. However, the two ribozymes differ in the nature of the general acid. 2'-Amino substitution experiments indicate that the general acid of the hammerhead ribozyme is the O2' of G8, while that of the pistol ribozyme is a hydrated metal ion. The two ribozymes are related but mechanistically distinct. | |||
Comparison of the Structures and Mechanisms of the Pistol and Hammerhead Ribozymes.,Wilson TJ, Liu Y, Li NS, Dai Q, Piccirilli JA, Lilley DMJ J Am Chem Soc. 2019 May 15;141(19):7865-7875. doi: 10.1021/jacs.9b02141. Epub, 2019 May 2. PMID:31017785<ref>PMID:31017785</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Lilley, D | <div class="pdbe-citations 6r47" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Lilley, D M.J]] | |||
[[Category: Liu, Y]] | [[Category: Liu, Y]] | ||
[[Category: Wilson, T M]] | |||
[[Category: Magnesium]] | |||
[[Category: Pseudoknot]] | |||
[[Category: Ribozyme]] | |||
[[Category: Rna]] | |||
Revision as of 06:02, 3 July 2019
The structure of pistol ribozyme bound to magnesium
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