SCP2-thiolase: Difference between revisions

Revision as of 12:58, 8 April 2019

Structure of the zebrafish SCP2-thiolase ^[1]

The SCP2-thiolase is a member of the thiolase family of enzymes^[1]. It has low sequence identity with any of the other thiolases. Like many other thiolases it is a dimer in solution. In the 6HSP crystal structure there are two monomers per asymmetric unit. One monomer forms the classical dimer with a two-fold related symmetry mate. The other monomer has a different packing and it is not forming the classical dimer: its structure is the structure of the monomer. There are large structural differences between these two monomers. The comparison of the structures of the "dimerised" monomer and the single monomer visualises the structural changes that happen when the single monomer dimerises to form the mature dimer.

The A-monomer is the single monomer, and the B-monomer is forming a dimer with its two-fold crystallographically related symmetry copy.

The active site is at the dimer interface

The active site is shaped by the residues of four loops

Function

Here something about function ^[2]

Disease

Relevance

Structural highlights

When the A and B monomers are compared the structural differences can best be seen in this morph.

This is a sample scene created with SAT to color by Group, and another to make a transparent representation of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

↑ Anbazhagan P, Harijan RK, Kiema TR, Janardan N, Murthy MR, Michels PA, Juffer AH, Wierenga RK. Phylogenetic relationships and classification of thiolases and thiolase-like proteins of Mycobacterium tuberculosis and Mycobacterium smegmatis. Tuberculosis (Edinb). 2014 Jul;94(4):405-12. doi: 10.1016/j.tube.2014.03.003., Epub 2014 Apr 4. PMID:24825023 doi:https://dx.doi.org/10.1016/j.tube.2014.03.003
↑ Venkatesan R, Sah-Teli SK, Awoniyi LO, Jiang G, Prus P, Kastaniotis AJ, Hiltunen JK, Wierenga RK, Chen Z. Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase. Nat Commun. 2014 Sep 9;5:4805. doi: 10.1038/ncomms5805. PMID:25203508 doi:https://dx.doi.org/10.1038/ncomms5805

References

↑ Kiema TR, Thapa CJ, Laitaoja M, Schmitz W, Maksimainen MM, Fukao T, Rouvinen J, Janis J, Wierenga RK. The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry. Biochem J. 2018 Dec 20. pii: BCJ20180788. doi: 10.1042/BCJ20180788. PMID:30573650 doi:https://dx.doi.org/10.1042/BCJ20180788

Proteopedia Page Contributors and Editors (what is this?)

Rik Wierenga, Michal Harel

[1] Anbazhagan P, Harijan RK, Kiema TR, Janardan N, Murthy MR, Michels PA, Juffer AH, Wierenga RK. Phylogenetic relationships and classification of thiolases and thiolase-like proteins of Mycobacterium tuberculosis and Mycobacterium smegmatis. Tuberculosis (Edinb). 2014 Jul;94(4):405-12. doi: 10.1016/j.tube.2014.03.003., Epub 2014 Apr 4. PMID:24825023 doi:https://dx.doi.org/10.1016/j.tube.2014.03.003

[2] Venkatesan R, Sah-Teli SK, Awoniyi LO, Jiang G, Prus P, Kastaniotis AJ, Hiltunen JK, Wierenga RK, Chen Z. Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase. Nat Commun. 2014 Sep 9;5:4805. doi: 10.1038/ncomms5805. PMID:25203508 doi:https://dx.doi.org/10.1038/ncomms5805

[1] Kiema TR, Thapa CJ, Laitaoja M, Schmitz W, Maksimainen MM, Fukao T, Rouvinen J, Janis J, Wierenga RK. The peroxisomal zebrafish SCP2-thiolase (type-1) is a weak transient dimer as revealed by crystal structures and native mass spectrometry. Biochem J. 2018 Dec 20. pii: BCJ20180788. doi: 10.1042/BCJ20180788. PMID:30573650 doi:https://dx.doi.org/10.1042/BCJ20180788

[1]

[2]

@@ Line 22: / Line 22: @@
 == Structural highlights ==
-<scene name='80/809821/Morph/1'>morph</scene>
+When the A and B monomers are compared the structural differences can best be seen in this <scene name='80/809821/morph-movie/1'>morph</scene>.
 This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

SCP2-thiolase: Difference between revisions

Revision as of 12:58, 8 April 2019

Structure of the zebrafish SCP2-thiolase ^[1]

Contents

Function

Disease

Relevance

Structural highlights

References

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

SCP2-thiolase: Difference between revisions

Revision as of 12:58, 8 April 2019

Structure of the zebrafish SCP2-thiolase [1]

Function

Disease

Relevance

Structural highlights

References

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Search

Structure of the zebrafish SCP2-thiolase ^[1]