2yx9: Difference between revisions

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[[Image:2yx9.jpg|left|200px]]
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{{STRUCTURE_2yx9|  PDB=2yx9  |  SCENE=  }}  
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'''Crystal structure of D298K copper amine oxidase from Arthrobacter globiformis'''
===Crystal structure of D298K copper amine oxidase from Arthrobacter globiformis===




==Overview==
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The biogenesis of the topaquinone (TPQ) cofactor of copper amine oxidase (CAO) is self-catalyzed and requires copper and molecular oxygen. A dopaquinone intermediate has been proposed to undergo 1,4-addition of a copper-associated water molecule to form the reduced form of TPQ (TPQ(red)), followed by facile oxidation by O(2) to yield the mature TPQ (TPQ(ox)). In this study, we have incorporated a lysine residue in the active site of Arthrobacter globiformis CAO (AGAO) by site-directed mutagenesis to produce D298K-AGAO. The X-ray crystal structure of D298K-AGAO at 1.7-A resolution revealed that a covalent linkage formed between the epsilon-amino side chain of Lys298 and the C2 position of a dopaquinone derived from Tyr382, a precursor to TPQ(ox). We assigned the species as an iminoquinone tautomer (LTI) of lysine tyrosylquinone (LTQ), the organic cofactor of lysyl oxidase (LOX). The time course of the formation of LTI at pH 6.8 was followed by UV/vis and resonance Raman spectroscopies. In the early phase of the reaction, an LTQ-like intermediate was observed. This intermediate then slowly converted to LTI in an isosbestic manner. Not only is the presence of a dopaquinone intermediate in the TPQ biogenesis confirmed, but it also provides strong support for the proposed intermediacy of a dopaquinone in the biogenesis of LTQ in LOX. Further, this study indicates that the dopaquinone intermediate in AGAO is mobile and can swing from the copper site into the active-site wedge to react with Lys298.
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==About this Structure==
==About this Structure==
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[[Category: Topaquinone]]
[[Category: Topaquinone]]
[[Category: Tpq]]
[[Category: Tpq]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:02:04 2008''

Revision as of 12:02, 28 July 2008

File:2yx9.png

Template:STRUCTURE 2yx9

Crystal structure of D298K copper amine oxidase from Arthrobacter globiformis

Template:ABSTRACT PUBMED 17715921

About this Structure

2YX9 is a Single protein structure of sequence from Arthrobacter globiformis. Full crystallographic information is available from OCA.

Reference

Trapping of a dopaquinone intermediate in the TPQ cofactor biogenesis in a copper-containing amine oxidase from Arthrobacter globiformis., Moore RH, Spies MA, Culpepper MB, Murakawa T, Hirota S, Okajima T, Tanizawa K, Mure M, J Am Chem Soc. 2007 Sep 19;129(37):11524-34. Epub 2007 Aug 23. PMID:17715921

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