4msv: Difference between revisions

Revision as of 10:06, 28 December 2022

Crystal structure of FASL and DcR3 complex

Structural highlights

4msv is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	GOL, MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TNF6B_HUMAN Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.^[1]

Publication Abstract from PubMed

The apoptotic effect of FasL:Fas signaling is disrupted by DcR3, a unique secreted member of the tumor necrosis factor receptor superfamily, which also binds and neutralizes TL1A and LIGHT. DcR3 is highly elevated in patients with various tumors and contributes to mechanisms by which tumor cells to evade host immune surveillance. Here we report the crystal structure of FasL in complex with DcR3. Comparison of FasL:DcR3 structure with our earlier TL1A:DcR3 and LIGHT:DcR3 structures supports a paradigm involving the recognition of invariant main-chain and conserved side-chain functionalities, which is responsible for the recognition of multiple TNF ligands exhibited by DcR3. The FasL:DcR3 structure also provides insight into the FasL:Fas recognition surface. We demonstrate that the ability of recombinant FasL to induce Jurkat cell apoptosis is significantly enhanced by native glycosylation or by structure-inspired mutations, both of which result in reduced tendency to aggregate. All of these activities are efficiently inhibited by recombinant DcR3.

Crystal Structure of the Complex of Human FasL and Its Decoy Receptor DcR3.,Liu W, Ramagopal U, Cheng H, Bonanno JB, Toro R, Bhosle R, Zhan C, Almo SC Structure. 2016 Nov 1;24(11):2016-2023. doi: 10.1016/j.str.2016.09.009. PMID:27806260^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhan C, Patskovsky Y, Yan Q, Li Z, Ramagopal U, Cheng H, Brenowitz M, Hui X, Nathenson SG, Almo SC. Decoy Strategies: The Structure of TL1A:DcR3 Complex. Structure. 2011 Feb 9;19(2):162-71. PMID:21300286 doi:10.1016/j.str.2010.12.004
↑ Liu W, Ramagopal U, Cheng H, Bonanno JB, Toro R, Bhosle R, Zhan C, Almo SC. Crystal Structure of the Complex of Human FasL and Its Decoy Receptor DcR3. Structure. 2016 Nov 1;24(11):2016-2023. doi: 10.1016/j.str.2016.09.009. PMID:27806260 doi:https://dx.doi.org/10.1016/j.str.2016.09.009

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Zhan C, Patskovsky Y, Yan Q, Li Z, Ramagopal U, Cheng H, Brenowitz M, Hui X, Nathenson SG, Almo SC. Decoy Strategies: The Structure of TL1A:DcR3 Complex. Structure. 2011 Feb 9;19(2):162-71. PMID:21300286 doi:10.1016/j.str.2010.12.004

[2] Liu W, Ramagopal U, Cheng H, Bonanno JB, Toro R, Bhosle R, Zhan C, Almo SC. Crystal Structure of the Complex of Human FasL and Its Decoy Receptor DcR3. Structure. 2016 Nov 1;24(11):2016-2023. doi: 10.1016/j.str.2016.09.009. PMID:27806260 doi:https://dx.doi.org/10.1016/j.str.2016.09.009

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='4msv' size='340' side='right'caption='[[4msv]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4msv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MSV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MSV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4msv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MSV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MSV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4en0|4en0]], [[3mhd|3mhd]], [[4j6g|4j6g]], [[4kgg|4kgg]], [[4kgq|4kgq]], [[3k51|3k51]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4msv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4msv OCA], [https://pdbe.org/4msv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4msv RCSB], [https://www.ebi.ac.uk/pdbsum/4msv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4msv ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TNFRSF6B, DCR3, TR6, UNQ186/PRO212 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), FASLG, APT1LG1, CD95L, FASL, TNFSF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4msv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4msv OCA], [http://pdbe.org/4msv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4msv RCSB], [http://www.ebi.ac.uk/pdbsum/4msv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4msv ProSAT]</span></td></tr>
 </table>
-== Disease ==
-[[http://www.uniprot.org/uniprot/TNFL6_HUMAN TNFL6_HUMAN]] Autoimmune lymphoproliferative syndrome. The disease is caused by mutations affecting the gene represented in this entry.
 == Function ==
-[[http://www.uniprot.org/uniprot/TNF6B_HUMAN TNF6B_HUMAN]] Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.<ref>PMID:21300286</ref>  [[http://www.uniprot.org/uniprot/TNFL6_HUMAN TNFL6_HUMAN]] Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.<ref>PMID:17557115</ref>   The FasL intracellular domain (FasL ICD) cytoplasmic form induces gene transcription inhibition.<ref>PMID:17557115</ref>
+[https://www.uniprot.org/uniprot/TNF6B_HUMAN TNF6B_HUMAN] Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.<ref>PMID:21300286</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 20: @@
 ==See Also==
-*[[Tumor necrosis factor ligand superfamily|Tumor necrosis factor ligand superfamily]]
+*[[Tumor necrosis factor ligand superfamily 3D structures|Tumor necrosis factor ligand superfamily 3D structures]]
-*[[Tumor necrosis factor receptor|Tumor necrosis factor receptor]]
+*[[Tumor necrosis factor receptor 3D structures|Tumor necrosis factor receptor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Almo, S C]]
+[[Category: Almo SC]]
-[[Category: Bhosle, R C]]
+[[Category: Bhosle RC]]
-[[Category: Bonanno, J B]]
+[[Category: Bonanno JB]]
-[[Category: IFN, Atoms-to-Animals:.The Immune Function Network]]
+[[Category: Liu W]]
-[[Category: Liu, W]]
+[[Category: Nathenson SG]]
-[[Category: Structural genomic]]
+[[Category: Ramagopal UA]]
-[[Category: Nathenson, S G]]
+[[Category: Zhan C]]
-[[Category: Ramagopal, U A]]
-[[Category: Zhan, C]]
-[[Category: Atoms-to-animals: the immune function network]]
-[[Category: Bind tnf receptor fa]]
-[[Category: Cd95l]]
-[[Category: Cytokine]]
-[[Category: Dcr3]]
-[[Category: Fas ligand]]
-[[Category: Fasl]]
-[[Category: Ifn]]
-[[Category: Immune system]]
-[[Category: Immunity]]
-[[Category: Jelly-roll fold]]
-[[Category: Membrane]]
-[[Category: Nysgrc]]
-[[Category: PSI, Protein structure initiative]]
-[[Category: Psi-biology]]
-[[Category: Research consortium]]
-[[Category: Secreted protein]]
-[[Category: Tnf]]
-[[Category: Tnf supe cd95l]]
-[[Category: Tnf superfamily]]
-[[Category: Tnf6]]
-[[Category: Tnfr]]

4msv: Difference between revisions

Revision as of 10:06, 28 December 2022

Crystal structure of FASL and DcR3 complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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