4q7o

The crystal structure of an immunity protein NMB0503 from Neisseria meningitidis MC58

spinqualitylabelsall models 4q7o, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4q7o is a 2 chain structure with sequence from Neisseria meningitidis MC58. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BR, FMT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q7DDN8_NEIMB Publication Abstract from PubMed Contact-dependent growth inhibition (CDI) is an important mechanism of intercellular competition between neighboring Gram-negative bacteria. CDI systems encode large surface-exposed CdiA effector proteins that carry a variety of C-terminal toxin domains (CdiA-CTs). All CDI(+) bacteria also produce CdiI immunity proteins that specifically bind to the cognate CdiA-CT and neutralize its toxin activity to prevent auto-inhibition. Here, the X-ray crystal structure of a CdiI immunity protein from Neisseria meningitidis MC58 is presented at 1.45 A resolution. The CdiI protein has structural homology to the Whirly family of RNA-binding proteins, but appears to lack the characteristic nucleic acid-binding motif of this family. Sequence homology suggests that the cognate CdiA-CT is related to the eukaryotic EndoU family of RNA-processing enzymes. A homology model is presented of the CdiA-CT based on the structure of the XendoU nuclease from Xenopus laevis. Molecular-docking simulations predict that the CdiA-CT toxin active site is occluded upon binding to the CdiI immunity protein. Together, these observations suggest that the immunity protein neutralizes toxin activity by preventing access to RNA substrates. The structure of a contact-dependent growth-inhibition (CDI) immunity protein from Neisseria meningitidis MC58.,Tan K, Johnson PM, Stols L, Boubion B, Eschenfeldt W, Babnigg G, Hayes CS, Joachimiak A, Goulding CW Acta Crystallogr F Struct Biol Commun. 2015 Jun 1;71(Pt 6):702-9. doi:, 10.1107/S2053230X15006585. Epub 2015 May 20. PMID:26057799 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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