6ris: Difference between revisions
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The | ==The Kb42S variant of the molybdenum storage protein== | ||
<StructureSection load='6ris' size='340' side='right'caption='[[6ris]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6ris]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RIS FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ris FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ris OCA], [http://pdbe.org/6ris PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ris RCSB], [http://www.ebi.ac.uk/pdbsum/6ris PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ris ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/MOSB_AZOVD MOSB_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule. [[http://www.uniprot.org/uniprot/MOSA_AZOVD MOSA_AZOVD]] Intracellular storage of molybdenum. Binds polyoxomolybdates. Can bind at least 90 molybdenum atoms per protein molecule. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The molybdenum storage protein (MoSto) deposits large amounts of molybdenum as polyoxomolybdate clusters in a heterohexameric (alphabeta)3 cage-like protein complex under ATP consumption. Here, we suggest a unique mechanism for the ATP-powered molybdate pumping process based on X-ray crystallography, cryoelectron microscopy, hydrogen-deuterium exchange mass spectrometry, and mutational studies of MoSto from Azotobacter vinelandii First, we show that molybdate, ATP, and Mg(2+) consecutively bind into the open ATP-binding groove of the beta-subunit, which thereafter becomes tightly locked by fixing the previously disordered N-terminal arm of the alpha-subunit over the beta-ATP. Next, we propose a nucleophilic attack of molybdate onto the gamma-phosphate of beta-ATP, analogous to the similar reaction of the structurally related UMP kinase. The formed instable phosphoric-molybdic anhydride becomes immediately hydrolyzed and, according to the current data, the released and accelerated molybdate is pressed through the cage wall, presumably by turning aside the Metbeta149 side chain. A structural comparison between MoSto and UMP kinase provides valuable insight into how an enzyme is converted into a molecular machine during evolution. The postulated direct conversion of chemical energy into kinetic energy via an activating molybdate kinase and an exothermic pyrophosphatase reaction to overcome a proteinous barrier represents a novelty in ATP-fueled biochemistry, because normally, ATP hydrolysis initiates large-scale conformational changes to drive a distant process. | |||
Molybdate pumping into the molybdenum storage protein via an ATP-powered piercing mechanism.,Brunle S, Eisinger ML, Poppe J, Mills DJ, Langer JD, Vonck J, Ermler U Proc Natl Acad Sci U S A. 2019 Dec 6. pii: 1913031116. doi:, 10.1073/pnas.1913031116. PMID:31811022<ref>PMID:31811022</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6ris" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Bruenle, S]] | |||
[[Category: Ermler, U]] | [[Category: Ermler, U]] | ||
[[Category: | [[Category: Amino acid kinase family]] | ||
[[Category: Atp hydrolysis]] | |||
[[Category: Metal binding protein]] | |||
[[Category: Mo storage]] | |||
[[Category: Polyoxomolybdate cluster]] | |||
