Structural highlights
4r5f is a 1 chain structure with sequence from Arcfu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | ACT, CA, CL, PG4, PMP |
| Related: | 4hvk, 4eb5, 4eb7 |
| Gene: | iscS2, AF_0564 (ARCFU) |
| Activity: | Cysteine desulfurase, with EC number 2.8.1.7 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[ISCS2_ARCFU] Catalyzes the removal of elemental sulfur from cysteine to produce alanine (By similarity).
Publication Abstract from PubMed
Iron sulfur ([Fe-S]) clusters are essential prosthetic groups involved in fundamental cell processes such as gene expression regulation, electron transfer and Lewis acid base chemistry. Central components of their biogenesis are pyridoxal-5'-phosphate (PLP) dependent l-cysteine desulfurases, which provide the necessary S atoms for [Fe-S] cluster assembly. The archaeon Archaeoglobus fulgidus (Af) has two ORFs, which although annotated as l-cysteine desulfurases of the ISC type (IscS), lack the essential Lys residue (K199 in Af) that forms a Schiff base with PLP. We have previously determined the structure of an Af(IscU-D35A-IscS)2 complex heterologously expressed in Escherichia coli and found it to contain a [Fe2S2] cluster. In order to understand the origin of sulfide in that structure we have performed a series of functional tests using wild type and mutated forms of AfIscS. In addition, we have determined the crystal structure of an AfIscS-D199K mutant. From these studies we conclude that: i) AfIscS has no desulfurase activity; ii) in our in vitro [Fe2S2] cluster assembly experiments, sulfide ions are non-enzymatically generated by a mixture of iron, l-cysteine and PLP and iii) the physiological role of AfIscS may be to provide a cysteine ligand to the nascent cluster as observed in the [Fe2S2]-Af(IscU-D35A-IscS)2 complex. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases.
IscS from Archaeoglobus fulgidus has no desulfurase activity but may provide a cysteine ligand for [FeS] cluster assembly.,Pagnier A, Nicolet Y, Fontecilla-Camps JC Biochim Biophys Acta. 2014 Oct 28. pii: S0167-4889(14)00374-7. doi:, 10.1016/j.bbamcr.2014.10.015. PMID:25447670[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Pagnier A, Nicolet Y, Fontecilla-Camps JC. IscS from Archaeoglobus fulgidus has no desulfurase activity but may provide a cysteine ligand for [FeS] cluster assembly. Biochim Biophys Acta. 2014 Oct 28. pii: S0167-4889(14)00374-7. doi:, 10.1016/j.bbamcr.2014.10.015. PMID:25447670 doi:https://dx.doi.org/10.1016/j.bbamcr.2014.10.015
