Sandbox Reserved 1546: Difference between revisions
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== Structural Highlights & Disease == | == Structural Highlights & Disease == | ||
<StructureSection load='1stp' size='340' side='right' caption='5H86 Structure' scene='80/806432/5h86_molecule/2'>Our enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Huamn Gcn5 enzyme bound to butyrl-coA can be summarized below: | <StructureSection load='1stp' size='340' side='right' caption='5H86 Structure' scene='80/806432/5h86_molecule/2'> | ||
<table><tr><td colspan='2'></td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCO:BUTYRYL+COENZYME+A'>BCO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone_acetyltransferase Histone acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.48 2.3.1.48] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5h86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h86 OCA], [http://pdbe.org/5h86 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h86 RCSB], [http://www.ebi.ac.uk/pdbsum/5h86 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h86 ProSAT]</span></td></tr> | |||
</table> | |||
Our enzyme, 5h86, can be expressed in ''E. coli'' and is found in humans <ref name = "Structural basis for acyl-group discrimination by human Gcn5L2"/>. The characteristics of this 5h86 Huamn Gcn5 enzyme bound to butyrl-coA can be summarized below: | |||
<p>• '''Found in These Organisms''': ''Homo sapiens''</p> | <p>• '''Found in These Organisms''': ''Homo sapiens''</p> | ||
<p>• '''Expressed in This System''': ''Escherichia coli''</p> | <p>• '''Expressed in This System''': ''Escherichia coli''</p> | ||
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<p>'''Protein Expression and Purification'''</p> | <p>'''Protein Expression and Purification'''</p> | ||
<p>• A plasmid encoding the His-tagged catalytic domain of human Gcn5L2 under T7 induction was obtained. The protein was expressed and purified. The purified protein was dialyzed into 20MM and concentrated to 9 mh ml- flash-frozen in liquid nitrogen and stored at -80 C.</p> | <p>• A plasmid encoding the His-tagged catalytic domain of human Gcn5L2 under T7 induction was obtained. The protein was expressed and purified. The purified protein was dialyzed into 20MM and concentrated to 9 mh ml- flash-frozen in liquid nitrogen and stored at -80 C.</p> | ||
== References == | == References == | ||
<references/> | <references/> | ||
