2bep: Difference between revisions

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[[Image:2bep.gif|left|200px]]
{{Seed}}
[[Image:2bep.png|left|200px]]


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{{STRUCTURE_2bep|  PDB=2bep  |  SCENE=  }}  
{{STRUCTURE_2bep|  PDB=2bep  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K'''
===CRYSTAL STRUCTURE OF UBIQUITIN CONJUGATING ENZYME E2-25K===




==Overview==
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Post-translational modification with small ubiquitin-related modifier (SUMO) alters the function of many proteins, but the molecular mechanisms and consequences of this modification are still poorly defined. During a screen for novel SUMO1 targets, we identified the ubiquitin-conjugating enzyme E2-25K (Hip2). SUMO attachment severely impairs E2-25K ubiquitin thioester and unanchored ubiquitin chain formation in vitro. Crystal structures of E2-25K(1-155) and of the E2-25K(1-155)-SUMO conjugate (E2-25K(*)SUMO) indicate that SUMO attachment interferes with E1 interaction through its location on the N-terminal helix. The SUMO acceptor site in E2-25K, Lys14, does not conform to the consensus site found in most SUMO targets (PsiKXE), and functions only in the context of an alpha-helix. In contrast, adjacent SUMO consensus sites are modified only when in unstructured peptides. The demonstration that secondary structure elements are part of SUMO attachment signals could contribute to a better prediction of SUMO targets.
The line below this paragraph, {{ABSTRACT_PUBMED_15723079}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 15723079 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15723079}}


==About this Structure==
==About this Structure==
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[[Category: Structural proteomics in europe]]
[[Category: Structural proteomics in europe]]
[[Category: Ubiquitin,e2 conjugating enzyme,protein degradation]]
[[Category: Ubiquitin,e2 conjugating enzyme,protein degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:28:17 2008''
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