6rnf: Difference between revisions

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'''Unreleased structure'''


The entry 6rnf is ON HOLD  until sometime in the future
==Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 30 ms timepoint==
<StructureSection load='6rnf' size='340' side='right'caption='[[6rnf]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RNF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Xylose_isomerase Xylose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.5 5.3.1.5] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rnf OCA], [http://pdbe.org/6rnf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rnf RCSB], [http://www.ebi.ac.uk/pdbsum/6rnf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rnf ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/XYLA_STRRU XYLA_STRRU]] Involved in D-xylose catabolism.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.


Authors: Mehrabi, P., Schulz, E.C., Miller, R.J.D.
Liquid application method for time-resolved analyses by serial synchrotron crystallography.,Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD Nat Methods. 2019 Oct;16(10):979-982. doi: 10.1038/s41592-019-0553-1. Epub 2019, Sep 16. PMID:31527838<ref>PMID:31527838</ref>


Description: Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Xylose Isomerase 30 ms timepoint
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
<div class="pdbe-citations 6rnf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Xylose isomerase]]
[[Category: Mehrabi, P]]
[[Category: Mehrabi, P]]
[[Category: Miller, R.J.D]]
[[Category: Miller, R J.D]]
[[Category: Schulz, E.C]]
[[Category: Schulz, E C]]
[[Category: Complex]]
[[Category: Diffusion]]
[[Category: Dynamic]]
[[Category: Isomerase]]
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