4uir: Difference between revisions

Revision as of 08:27, 22 March 2023

Structure of oleate hydratase from Elizabethkingia meningoseptica

Structural highlights

4uir is a 2 chain structure with sequence from Elizabethkingia meningoseptica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	FAD, NA, P6G, PO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

OLHYD_ELIME

Publication Abstract from PubMed

Hydratases provide access to secondary and tertiary alcohols by regio- and/or stereospecifically adding water to carbon-carbon double bonds. Thereby, hydroxyl groups are introduced without the need for costly co-factor recycling, which renders this approach highly interesting on an industrial scale. Here, we present the first crystal structure of a recombinant oleate hydratase originating from Elizabethkingia meningoseptica in the presence of the flavin cofactor (FAD). A structure-based mutagenesis study targeting active site residues identified E122 and Y241 as crucial for the activation of a water molecule and protonation of the double bond, respectively. Moreover, we also observed that two-electron reduction of FAD results in a 7-fold increase of substrate hydration rate. We propose the first reaction mechanism for this enzyme class that rationalizes the requirement of flavin cofactor and the involvement of conserved amino acid residues in this regio- and stereoselective hydration.

Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica.,Engleder M, Pavkov-Keller T, Emmerstorfer A, Hromic A, Schrempf S, Steinkellner G, Wriessnegger T, Leitner E, Strohmeier GA, Kaluzna I, Mink D, Schurmann M, Wallner S, Macheroux P, Gruber K, Pichler H Chembiochem. 2015 Jun 15. doi: 10.1002/cbic.201500269. PMID:26077980^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Engleder M, Pavkov-Keller T, Emmerstorfer A, Hromic A, Schrempf S, Steinkellner G, Wriessnegger T, Leitner E, Strohmeier GA, Kaluzna I, Mink D, Schurmann M, Wallner S, Macheroux P, Gruber K, Pichler H. Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica. Chembiochem. 2015 Jun 15. doi: 10.1002/cbic.201500269. PMID:26077980 doi:https://dx.doi.org/10.1002/cbic.201500269

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OCA

[1] Engleder M, Pavkov-Keller T, Emmerstorfer A, Hromic A, Schrempf S, Steinkellner G, Wriessnegger T, Leitner E, Strohmeier GA, Kaluzna I, Mink D, Schurmann M, Wallner S, Macheroux P, Gruber K, Pichler H. Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica. Chembiochem. 2015 Jun 15. doi: 10.1002/cbic.201500269. PMID:26077980 doi:https://dx.doi.org/10.1002/cbic.201500269

[1]

@@ Line 3: / Line 3: @@
 <StructureSection load='4uir' size='340' side='right'caption='[[4uir]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4uir]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_13253 Atcc 13253]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UIR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UIR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4uir]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UIR FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uic|4uic]], [[4uid|4uid]], [[4uie|4uie]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uir OCA], [https://pdbe.org/4uir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uir RCSB], [https://www.ebi.ac.uk/pdbsum/4uir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uir ProSAT]</span></td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oleate_hydratase Oleate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.53 4.2.1.53] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4uir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uir OCA], [http://pdbe.org/4uir PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4uir RCSB], [http://www.ebi.ac.uk/pdbsum/4uir PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4uir ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/OLHYD_ELIME OLHYD_ELIME]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 13253]]
+[[Category: Elizabethkingia meningoseptica]]
 [[Category: Large Structures]]
-[[Category: Oleate hydratase]]
+[[Category: Emmerstorfer A]]
-[[Category: Emmerstorfer, A]]
+[[Category: Engleder M]]
-[[Category: Engleder, M]]
+[[Category: Gruber K]]
-[[Category: Gruber, K]]
+[[Category: Hromic A]]
-[[Category: Hromic, A]]
+[[Category: Kaluzna I]]
-[[Category: Kaluzna, I]]
+[[Category: Leitner E]]
-[[Category: Leitner, E]]
+[[Category: Macheroux P]]
-[[Category: Macheroux, P]]
+[[Category: Mink D]]
-[[Category: Mink, D]]
+[[Category: Pavkov-Keller T]]
-[[Category: Pavkov-Keller, T]]
+[[Category: Pichler H]]
-[[Category: Pichler, H]]
+[[Category: Schrempf S]]
-[[Category: Schrempf, S]]
+[[Category: Schuermann M]]
-[[Category: Schuermann, M]]
+[[Category: Steinkellner G]]
-[[Category: Steinkellner, G]]
+[[Category: Strohmeier GA]]
-[[Category: Strohmeier, G A]]
+[[Category: Wallner S]]
-[[Category: Wallner, S]]
+[[Category: Wriessnegger T]]
-[[Category: Wriessnegger, T]]
-[[Category: Lyase]]

4uir: Difference between revisions

Revision as of 08:27, 22 March 2023

Structure of oleate hydratase from Elizabethkingia meningoseptica

Structural highlights

Function

Publication Abstract from PubMed

References

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