6rim: Difference between revisions

Revision as of 23:15, 5 June 2019

Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin

Structural highlights

6rim is a 8 chain structure with sequence from Dsm 25784. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CA, ZN
NonStd Res:	MSE
Gene:	WOSG25_110680 (DSM 25784)
Activity:	Bontoxilysin, with EC number 3.4.24.69
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BXWO_WEIOS] When overexpressed the N-terminus (residues 1-476) cleaves rat synaptobrevin-2/VAMP2 between '89-Trp-|-Trp-90' in vitro. This releases the cytoplasmic domain of VAMP2 from the synaptic vesicle membrane, which would prevent the assembly of the trans-SNARE complex on the membrane and thus prevent vesicle-target membrane fusion and neurotransmitter release (PubMed:27443638).^[1]

Publication Abstract from PubMed

Botulinum neurotoxins (BoNTs) are the most potent toxins known. So far, eight serotypes have been identified that all act as zinc-dependent endopeptidases targeting SNARE proteins and inhibiting the release of neurotransmitters. Recently, the first botulinum toxin-like protein was identified outside the Clostridial genus, designated BoNT/Wo in the genome of Weissella oryzae. Here, we report the 1.6 A X-ray crystal structure of the light chain of BoNT/Wo (LC/Wo). LC/Wo presents the core fold common to BoNTs but has an unusually wide, open, and negatively charged catalytic pocket, with an additional Ca(2+) ion besides the zinc ion and a unique ss-hairpin motif. The structural information will help establish the substrate profile of BoNT/Wo and help our understanding of how BoNT evolved. This article is protected by copyright. All rights reserved.

Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin.,Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zornetta I, Azarnia Tehran D, Arrigoni G, Anniballi F, Bano L, Leka O, Zanotti G, Binz T, Montecucco C. The first non Clostridial botulinum-like toxin cleaves VAMP within the juxtamembrane domain. Sci Rep. 2016 Jul 22;6:30257. doi: 10.1038/srep30257. PMID:27443638 doi:https://dx.doi.org/10.1038/srep30257
↑ Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P. Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin. FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466 doi:https://dx.doi.org/10.1002/1873-3468.13446

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OCA

[1] Zornetta I, Azarnia Tehran D, Arrigoni G, Anniballi F, Bano L, Leka O, Zanotti G, Binz T, Montecucco C. The first non Clostridial botulinum-like toxin cleaves VAMP within the juxtamembrane domain. Sci Rep. 2016 Jul 22;6:30257. doi: 10.1038/srep30257. PMID:27443638 doi:https://dx.doi.org/10.1038/srep30257

[2] Kosenina S, Masuyer G, Zhang S, Dong M, Stenmark P. Crystal structure of the catalytic domain of the Weissela oryzae botulinum-like toxin. FEBS Lett. 2019 May 20. doi: 10.1002/1873-3468.13446. PMID:31111466 doi:https://dx.doi.org/10.1002/1873-3468.13446

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='6rim' size='340' side='right'caption='[[6rim]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6rim]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RIM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6rim]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_25784 Dsm 25784]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RIM FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WOSG25_110680 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1129792 DSM 25784])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Bontoxilysin Bontoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.69 3.4.24.69] </span></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6rim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rim OCA], [http://pdbe.org/6rim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rim RCSB], [http://www.ebi.ac.uk/pdbsum/6rim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rim ProSAT]</span></td></tr>
@@ Line 25: / Line 26: @@
 </StructureSection>
 [[Category: Bontoxilysin]]
+[[Category: Dsm 25784]]
 [[Category: Large Structures]]
 [[Category: Kosenina, S]]

6rim: Difference between revisions

Revision as of 23:15, 5 June 2019

Crystal structure of the catalytic domain of the Weissela oryzae botulinum like toxin

Structural highlights

Function

Publication Abstract from PubMed

References

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