Sandbox Reserved 1482: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 56: | Line 56: | ||
• The <scene name='80/802656/Light_chain/2'>light chain</scene> has a molecular weight of 80 kDa and is composed of 684 amino acids <ref name="Binhoreau" />. It contains two domains: a unique A domain of 371 amino acids and a duplicated C domain of 153 amino acids and 160 amino acids, respectively <ref name="Binhoreau" />. These domains are ranked in the following order A<sub>3</sub>-C<sub>1</sub>-C<sub>2</sub> <ref name="wikipedia" /><ref name="Binhoreau" />. It is composed of 42 % irregular structure, 36 % β-strands, and 22 % α-helices <ref name="Binhoreau" />. The C<sub>1</sub> and C<sub>2</sub> domains are defined by a distorted β barrel, while A<sub>3</sub>, as well as A<sub>1</sub> and A<sub>2</sub>, is composed of two connected β barrels <ref name="Ngo" />. This chain also contains of the major binding site of von Willebrand Factor at its N-terminus <ref name="Binhoreau" />. | • The <scene name='80/802656/Light_chain/2'>light chain</scene> has a molecular weight of 80 kDa and is composed of 684 amino acids <ref name="Binhoreau" />. It contains two domains: a unique A domain of 371 amino acids and a duplicated C domain of 153 amino acids and 160 amino acids, respectively <ref name="Binhoreau" />. These domains are ranked in the following order A<sub>3</sub>-C<sub>1</sub>-C<sub>2</sub> <ref name="wikipedia" /><ref name="Binhoreau" />. It is composed of 42 % irregular structure, 36 % β-strands, and 22 % α-helices <ref name="Binhoreau" />. The C<sub>1</sub> and C<sub>2</sub> domains are defined by a distorted β barrel, while A<sub>3</sub>, as well as A<sub>1</sub> and A<sub>2</sub>, is composed of two connected β barrels <ref name="Ngo" />. This chain also contains of the major binding site of von Willebrand Factor at its N-terminus <ref name="Binhoreau" />. | ||
Figure 1: Domain organisation of the uncleaved coagulation factor VIII (top) and the engineered factor without B domain (bottom). (Figure adapted from <ref name="Ngo" />) | |||
[[Image:Domain organisation factorVIII (un)cleaved.png]] | |||
Both chains are non-covalently associated through to a calcium ion to form the active heterodimer <ref name="Ngo" /><ref name="Binhoreau" />. This complex is the pro-coagulant factor VIIIa <ref name="wikipedia" />. | Both chains are non-covalently associated through to a calcium ion to form the active heterodimer <ref name="Ngo" /><ref name="Binhoreau" />. This complex is the pro-coagulant factor VIIIa <ref name="wikipedia" />. | ||
