4za1

Crystal Structure of NosA Involved in Nosiheptide Biosynthesis

spinqualitylabelsall models 4za1, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4za1 is a 3 chain structure with sequence from Streptomyces actuosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: DTT Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C6FX40_STRAS Publication Abstract from PubMed Nosiheptide is a parent compound of thiopeptide family that exhibit potent activities against various bacterial pathogens. Its C-terminal amide formation is catalyzed by NosA, which is an unusual strategy for maturating certain thiopeptides by processing their precursor peptides featuring a serine extension. We here report the crystal structure of truncated NosA1-111 variant, revealing three key elements, including basic lysine 49 (K49), acidic glutamic acid 101 (E101) and flexible C-terminal loop NosA112-151, are crucial to the catalytic terminal amide formation in nosiheptide biosynthesis. The side-chain of residue K49 and the C-terminal loop fasten the substrate through hydrogen bonds and hydrophobic interactions. The side-chain of residue E101 enhances nucleophilic attack of H2O to the methyl imine intermediate, leading to Calpha-N bond cleavage and nosiheptide maturation. The sequence alignment of NosA and its homologs NocA, PbtH, TpdK and BerI, and the enzymatic assay suggest that the mechanistic studies on NosA present an intriguing paradigm about how NosA family members function during thiopeptide biosynthesis. Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis.,Liu S, Guo H, Zhang T, Han L, Yao P, Zhang Y, Rong N, Yu Y, Lan W, Wang C, Ding J, Wang R, Liu W, Cao C Sci Rep. 2015 Aug 5;5:12744. doi: 10.1038/srep12744. PMID:26244829 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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