4zra: Difference between revisions

Revision as of 07:38, 18 May 2023

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LPRG BINDING TO TRIACYLGLYCERIDE

Structural highlights

4zra is a 2 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	4RF
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LPRG_MYCTU TLR2 agonist that can inhibit primary human macrophage MHC-II Ag processing. Required for Rv1410c (P55) activity.^[1] ^[2]

Publication Abstract from PubMed

Mycobacterium tuberculosis (Mtb) mutants lacking rv1411c, which encodes the lipoprotein LprG, and rv1410c, which encodes a putative efflux pump, are dramatically attenuated for growth in mice. Here we show that loss of LprG-Rv1410 in Mtb leads to intracellular triacylglyceride (TAG) accumulation, and overexpression of the locus increases the levels of TAG in the culture medium, demonstrating a role of this locus in TAG transport. LprG binds TAG within a large hydrophobic cleft and is sufficient to transfer TAG from donor to acceptor membranes. Further, LprG-Rv1410 is critical for broadly regulating bacterial growth and metabolism in vitro during carbon restriction and in vivo during infection of mice. The growth defect in mice is due to disrupted bacterial metabolism and occurs independently of key immune regulators. The in vivo essentiality of this locus suggests that this export system and other regulators of metabolism should be considered as targets for novel therapeutics.

Mycobacterial Metabolic Syndrome: LprG and Rv1410 Regulate Triacylglyceride Levels, Growth Rate and Virulence in Mycobacterium tuberculosis.,Martinot AJ, Farrow M, Bai L, Layre E, Cheng TY, Tsai JH, Iqbal J, Annand JW, Sullivan ZA, Hussain MM, Sacchettini J, Moody DB, Seeliger JC, Rubin EJ PLoS Pathog. 2016 Jan 11;12(1):e1005351. doi: 10.1371/journal.ppat.1005351., eCollection 2016 Jan. PMID:26751071^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gehring AJ, Dobos KM, Belisle JT, Harding CV, Boom WH. Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J Immunol. 2004 Aug 15;173(4):2660-8. PMID:15294983
↑ Farrow MF, Rubin EJ. Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein. J Bacteriol. 2008 Mar;190(5):1783-91. Epub 2007 Dec 21. PMID:18156250 doi:10.1128/JB.01046-07
↑ Martinot AJ, Farrow M, Bai L, Layre E, Cheng TY, Tsai JH, Iqbal J, Annand JW, Sullivan ZA, Hussain MM, Sacchettini J, Moody DB, Seeliger JC, Rubin EJ. Mycobacterial Metabolic Syndrome: LprG and Rv1410 Regulate Triacylglyceride Levels, Growth Rate and Virulence in Mycobacterium tuberculosis. PLoS Pathog. 2016 Jan 11;12(1):e1005351. doi: 10.1371/journal.ppat.1005351., eCollection 2016 Jan. PMID:26751071 doi:https://dx.doi.org/10.1371/journal.ppat.1005351

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Gehring AJ, Dobos KM, Belisle JT, Harding CV, Boom WH. Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J Immunol. 2004 Aug 15;173(4):2660-8. PMID:15294983

[2] Farrow MF, Rubin EJ. Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein. J Bacteriol. 2008 Mar;190(5):1783-91. Epub 2007 Dec 21. PMID:18156250 doi:10.1128/JB.01046-07

[3] Martinot AJ, Farrow M, Bai L, Layre E, Cheng TY, Tsai JH, Iqbal J, Annand JW, Sullivan ZA, Hussain MM, Sacchettini J, Moody DB, Seeliger JC, Rubin EJ. Mycobacterial Metabolic Syndrome: LprG and Rv1410 Regulate Triacylglyceride Levels, Growth Rate and Virulence in Mycobacterium tuberculosis. PLoS Pathog. 2016 Jan 11;12(1):e1005351. doi: 10.1371/journal.ppat.1005351., eCollection 2016 Jan. PMID:26751071 doi:https://dx.doi.org/10.1371/journal.ppat.1005351

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='4zra' size='340' side='right'caption='[[4zra]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zra]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZRA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zra]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZRA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZRA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=4RF:TRIPALMITOYLGLYCEROL'>4RF</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4RF:TRIPALMITOYLGLYCEROL'>4RF</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3mh8|3mh8]], [[3mha|3mha]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zra OCA], [https://pdbe.org/4zra PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zra RCSB], [https://www.ebi.ac.uk/pdbsum/4zra PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zra ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lprG, lpp-27, Rv1411c, MTCY21B4.28c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zra OCA], [http://pdbe.org/4zra PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zra RCSB], [http://www.ebi.ac.uk/pdbsum/4zra PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zra ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LPRG_MYCTU LPRG_MYCTU]] TLR2 agonist that can inhibit primary human macrophage MHC-II Ag processing. Required for Rv1410c (P55) activity.<ref>PMID:15294983</ref> <ref>PMID:18156250</ref>
+[https://www.uniprot.org/uniprot/LPRG_MYCTU LPRG_MYCTU] TLR2 agonist that can inhibit primary human macrophage MHC-II Ag processing. Required for Rv1410c (P55) activity.<ref>PMID:15294983</ref> <ref>PMID:18156250</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Myctu]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-[[Category: Annand, J]]
+[[Category: Annand J]]
-[[Category: Bai, L]]
+[[Category: Bai L]]
-[[Category: Cheng, T Y]]
+[[Category: Cheng TY]]
-[[Category: Farrow, M]]
+[[Category: Farrow M]]
-[[Category: Hussain, M]]
+[[Category: Hussain M]]
-[[Category: Iqbal, J]]
+[[Category: Iqbal J]]
-[[Category: Layre, E]]
+[[Category: Layre E]]
-[[Category: Martinot, A J]]
+[[Category: Martinot AJ]]
-[[Category: Moody, D B]]
+[[Category: Moody DB]]
-[[Category: Rubin, E J]]
+[[Category: Rubin EJ]]
-[[Category: Sacchettini, J]]
+[[Category: Sacchettini J]]
-[[Category: Seeliger, J]]
+[[Category: Seeliger J]]
-[[Category: Sullivan, Z]]
+[[Category: Sullivan Z]]
-[[Category: Structural genomic]]
+[[Category: Tsai JHC]]
-[[Category: Tsai, J H.C]]
-[[Category: Lipid binding protein]]
-[[Category: Lipoprotein]]
-[[Category: Lprg]]
-[[Category: Tbsgc]]

4zra: Difference between revisions

Revision as of 07:38, 18 May 2023

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LPRG BINDING TO TRIACYLGLYCERIDE

Structural highlights

Function

Publication Abstract from PubMed

References

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