6r7m: Difference between revisions
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r7m OCA], [http://pdbe.org/6r7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r7m RCSB], [http://www.ebi.ac.uk/pdbsum/6r7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r7m ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r7m OCA], [http://pdbe.org/6r7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r7m RCSB], [http://www.ebi.ac.uk/pdbsum/6r7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r7m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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== Publication Abstract from PubMed == | |||
High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the several thousand to a few million protein particles required for structure determination by cryogenic electron microscopy (cryo-EM) can be provided by miniaturized systems. Here, we present a microfluidic method for the rapid isolation of a target protein and its direct preparation for cryo-EM. Less than 1 muL of cell lysate is required as starting material to solve the atomic structure of the untagged, endogenous human 20S proteasome. Our work paves the way for high-throughput structure determination of proteins from minimal amounts of cell lysate and opens more opportunities for the isolation of sensitive, endogenous protein complexes. | |||
Microfluidic protein isolation and sample preparation for high-resolution cryo-EM.,Schmidli C, Albiez S, Rima L, Righetto R, Mohammed I, Oliva P, Kovacik L, Stahlberg H, Braun T Proc Natl Acad Sci U S A. 2019 Jul 10. pii: 1907214116. doi:, 10.1073/pnas.1907214116. PMID:31292253<ref>PMID:31292253</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 6r7m" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | </StructureSection> | ||
