6s5a: Difference between revisions

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'''Unreleased structure'''


The entry 6s5a is ON HOLD
==CRYSTAL STRUCTURE OF FC P329G LALA WITH ANTI FC P329G FAB==
<StructureSection load='6s5a' size='340' side='right'caption='[[6s5a]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6s5a]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S5A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s5a OCA], [http://pdbe.org/6s5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s5a RCSB], [http://www.ebi.ac.uk/pdbsum/6s5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s5a ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monoclonal antibody-based therapeutics are an integral part of treatment of different human diseases, and the selection of suitable antibody candidates during the discovery phase is essential. Here, we describe a novel, cellular screening approach for the identification and characterization of therapeutic antibodies suitable for conversion into T cell bispecific antibodies using chimeric antigen receptor (CAR) transduced Jurkat-NFAT-luciferase reporter cells (CAR-J). For that purpose, we equipped a Jurkat-NFAT reporter cell line with a universal CAR, based on a monoclonal antibody recognizing the P329G mutation in the Fc-part of effector-silenced human IgG1-antibodies. In addition to scFv-based second generation CARs, Fab-based CARs employing the P329G-binder were generated. Using these anti-P329G-CAR-J cells together with the respective P329G-mutated IgG1-antibodies, we established a system, which facilitates the rapid testing of therapeutic antibody candidates in a flexible, high throughput setting during early stage discovery. We show that both, scFv- and Fab-based anti-P329G-CAR-J cells elicit a robust and dose-dependent luciferase signal if the respective antibody acts as an adaptor between tumor target and P329G-CAR-J cells. Importantly, we could demonstrate that functional characteristics of the antibody candidates, derived from the anti-P329G-CAR-J screening assay, are predictive for the functionality of these antibodies in the T cell bispecific antibody format.


Authors: Ehler, A., Darowski, D., Jost, C., Stubenrauch, K., Wessels, U., Benz, J., Birk, M., Freimoser-Grundschober, A., Bruenker, P., Moessner, E., Umana, P., Kobold, S., Klein, C.
P329G-CAR-J: a novel Jurkat-NFAT-based CAR-T reporter system recognizing the P329G Fc mutation.,Darowski D, Jost C, Stubenrauch K, Wessels U, Benz J, Ehler A, Freimoser-Grundschober A, Brunker P, Mossner E, Umana P, Kobold S, Klein C Protein Eng Des Sel. 2019 Dec 31;32(5):207-218. doi: 10.1093/protein/gzz027. PMID:31504896<ref>PMID:31504896</ref>


Description: CRYSTAL STRUCTURE OF FC P329G LALA WITH ANTI FC P329G FAB
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Kobold, S]]
<div class="pdbe-citations 6s5a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Large Structures]]
[[Category: Lk3 transgenic mice]]
[[Category: Benz, J]]
[[Category: Birk, M]]
[[Category: Bruenker, P]]
[[Category: Darowski, D]]
[[Category: Ehler, A]]
[[Category: Ehler, A]]
[[Category: Wessels, U]]
[[Category: Freimoser-Grundschober, A]]
[[Category: Jost, C]]
[[Category: Klein, C]]
[[Category: Klein, C]]
[[Category: Jost, C]]
[[Category: Kobold, S]]
[[Category: Moessner, E]]
[[Category: Moessner, E]]
[[Category: Stubenrauch, K]]
[[Category: Umana, P]]
[[Category: Umana, P]]
[[Category: Birk, M]]
[[Category: Wessels, U]]
[[Category: Freimoser-Grundschober, A]]
[[Category: Anti p329g lala fab]]
[[Category: Stubenrauch, K]]
[[Category: Antibody]]
[[Category: Benz, J]]
[[Category: Fab]]
[[Category: Darowski, D]]
[[Category: Fc]]
[[Category: Bruenker, P]]
[[Category: Immune system]]
[[Category: P329g lala]]
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