2qqu: Difference between revisions

From Proteopedia
Jump to navigationJump to search
Newer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
New page: left|200px <!-- The line below this paragraph, containing "STRUCTURE_2qqu", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
Line 1: Line 1:
[[Image:2qqu.jpg|left|200px]]
{{Seed}}
[[Image:2qqu.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2qqu|  PDB=2qqu  |  SCENE=  }}  
{{STRUCTURE_2qqu|  PDB=2qqu  |  SCENE=  }}  


'''Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose'''
===Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose===




==Overview==
<!--  
In plants, cell-wall invertases fulfil important roles in carbohydrate partitioning, growth, development and crop yield. In this study, we report on different X-ray crystal structures of Arabidopsis thaliana cell-wall invertase 1 (AtcwINV1) mutants with sucrose. These structures reveal a detailed view of sucrose binding in the active site of the wild-type AtcwINV1. Compared to related enzyme-sucrose complexes, important differences in the orientation of the glucose subunit could be observed. The structure of the E203Q AtcwINV1 mutant showed a complete new binding modus, whereas the D23A, E203A and D239A structures most likely represent the productive binding modus. Together with a hydrophobic zone formed by the conserved W20, W47 and W82, the residues N22, D23, R148, E203, D149 and D239 are necessary to create the ideal sucrose-binding pocket. D239 can interact directly with the glucose moiety of sucrose, whereas K242 has an indirect role in substrate stabilization. Most probably, K242 keeps D239 in a favourable position upon substrate binding. Unravelling the exact position of sucrose in plant cell-wall invertases is a necessary step towards the rational design of superior invertases to further increase crop yield and biomass production.
The line below this paragraph, {{ABSTRACT_PUBMED_18258263}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 18258263 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_18258263}}


==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Invertase]]
[[Category: Invertase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:41:30 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:37:33 2008''

Revision as of 15:37, 27 July 2008

File:2qqu.png

Template:STRUCTURE 2qqu

Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose

Template:ABSTRACT PUBMED 18258263

About this Structure

2QQU is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Crystal structures of Arabidopsis thaliana cell-wall invertase mutants in complex with sucrose., Lammens W, Le Roy K, Van Laere A, Rabijns A, Van den Ende W, J Mol Biol. 2008 Mar 21;377(2):378-85. Epub 2008 Jan 5. PMID:18258263

Page seeded by OCA on Sun Jul 27 18:37:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2qqu&oldid=661040"