2bx4: Difference between revisions

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<StructureSection load='2bx4' size='340' side='right'caption='[[2bx4]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
<StructureSection load='2bx4' size='340' side='right'caption='[[2bx4]], [[Resolution|resolution]] 2.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2bx4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus_sin2774 Sars coronavirus sin2774]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BX4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2bx4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sars_coronavirus_sin2774 Sars coronavirus sin2774]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BX4 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1o5s|1o5s]], [[1p4x|1p4x]], [[1p76|1p76]], [[1p9s|1p9s]], [[1p9t|1p9t]], [[1p9u|1p9u]], [[1pa5|1pa5]], [[1puk|1puk]], [[1q1x|1q1x]], [[1q2w|1q2w]], [[1q4z|1q4z]], [[1qz8|1qz8]], [[1ssk|1ssk]], [[1sxf|1sxf]], [[1uj1|1uj1]], [[1uk2|1uk2]], [[1uk3|1uk3]], [[1uk4|1uk4]], [[1uw7|1uw7]], [[1wnc|1wnc]], [[1xak|1xak]], [[1xjp|1xjp]], [[1xjr|1xjr]], [[2bw6|2bw6]], [[2bx3|2bx3]]</td></tr>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1o5s|1o5s]], [[1p4x|1p4x]], [[1p76|1p76]], [[1p9s|1p9s]], [[1p9t|1p9t]], [[1p9u|1p9u]], [[1pa5|1pa5]], [[1puk|1puk]], [[1q1x|1q1x]], [[1q2w|1q2w]], [[1q4z|1q4z]], [[1qz8|1qz8]], [[1ssk|1ssk]], [[1sxf|1sxf]], [[1uj1|1uj1]], [[1uk2|1uk2]], [[1uk3|1uk3]], [[1uk4|1uk4]], [[1uw7|1uw7]], [[1wnc|1wnc]], [[1xak|1xak]], [[1xjp|1xjp]], [[1xjr|1xjr]], [[2bw6|2bw6]], [[2bx3|2bx3]]</div></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bx4 OCA], [http://pdbe.org/2bx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bx4 RCSB], [http://www.ebi.ac.uk/pdbsum/2bx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bx4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bx4 OCA], [https://pdbe.org/2bx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bx4 RCSB], [https://www.ebi.ac.uk/pdbsum/2bx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bx4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>   
[[https://www.uniprot.org/uniprot/R1A_CVHSA R1A_CVHSA]] The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>  Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref>   
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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==See Also==
==See Also==
*[[SARS Coronavirus Main Proteinase|SARS Coronavirus Main Proteinase]]
*[[Virus protease 3D structures|Virus protease 3D structures]]
== References ==
== References ==
<references/>
<references/>
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