1vec: Difference between revisions

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[[Image:1vec.jpg|left|200px]]
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[[Image:1vec.png|left|200px]]


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{{STRUCTURE_1vec|  PDB=1vec  |  SCENE=  }}  
{{STRUCTURE_1vec|  PDB=1vec  |  SCENE=  }}  


'''Crystal structure of the N-terminal domain of rck/p54, a human DEAD-box protein'''
===Crystal structure of the N-terminal domain of rck/p54, a human DEAD-box protein===




==Overview==
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Human rck/p54, a product of the gene cloned at the breakpoint of t(11; 14) (q23;q32) chromosomal translocation on 11q23 in B-cell lymphoma, is a member of the DEAD-box RNA helicase family. Here, the crystal structure of Nc-rck/p54, the N-terminal core domain of rck/p54, revealed that the P-loop in motif I formed a closed conformation, which was induced by Asn131, a residue unique to the RCK subfamily. It appears that ATP does not bind to the P-loop. The results of dynamic light scattering revealed to ATP-induced conformational change of rck/p54. It was demonstrated that free rck/p54 is a distended molecule in solution, and that the approach between N-terminal core and C-terminal domains for ATP binding would be essential when unwinding RNA. The results from helicase assay using electron micrograph, ATP hydrolytic and luciferase assay showed that c-myc IRES RNA, whose secondary structure regulates IRES-dependant translation, was unwound by rck/p54 and indicated that it is a good substrate for rck/p54. Over-expression of rck/p54 in HeLa cells caused growth inhibition and cell cycle arrest at G2/M with down-regulation of c-myc expression. These findings altogether suggest that rck/p54 may affect the IRES-dependent translation of c-myc even in the cells.
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{{ABSTRACT_PUBMED_16611246}}


==About this Structure==
==About this Structure==
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[[Category: Rna binding protein]]
[[Category: Rna binding protein]]
[[Category: Rna helicase]]
[[Category: Rna helicase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:21:11 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:24:41 2008''

Revision as of 21:24, 27 July 2008

File:1vec.png

Template:STRUCTURE 1vec

Crystal structure of the N-terminal domain of rck/p54, a human DEAD-box protein

Template:ABSTRACT PUBMED 16611246

About this Structure

1VEC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes., Matsui T, Hogetsu K, Usukura J, Sato T, Kumasaka T, Akao Y, Tanaka N, Genes Cells. 2006 Apr;11(4):439-52. PMID:16611246

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